Rajalakshmi, Kanagaraj Nam, Yun-Sik Selvaraj, Muthusamy Lee, Yeonhee Lee, Kang-Bong 2024-01-19T23:01:43Z 2024-01-19T23:01:43Z 2021-09-03 2018-04-15 0925-4005 https://pubs.kist.re.kr/handle/201004/121475 A boronate group integrated on a triphenylimidazoleoxadiazolephenyl (TPIOP) moiety exhibited unusual sensing and detection abilities towards citrate. TPIOP boronate exhibited a bathochromic shift in its fluorescence emission upon interaction with citrate. Quantification of trace levels of citrate ions in the human body may aid in the diagnosis of prostate cancer and kidney malfunctions. Herein, a novel, metal free sensing strategy for citrate was developed based on fluorimetric detection with TPIOP boronate. The fluorescence of TPIOP boronate increased linearly and was gradually red-shifted upon addition of citrate. The enhanced emission intensity was attributed to aggregation induced emission. The vacant 'p' orbital of boron in TPIOP boronate acts as a Lewis acid, and accepts electrons from citrate, which is a Lewis base. Upon binding, the hybridization geometry of boron changes from sp(2) trigonal planar to sp(3) tetrahedral. The TPIOP boronate-citrate aggregates increase the polarity of TPIOP-boronate, which caused the red shift in the fluorescence. Citrate could be detected visually using a UV lamp, as the probe changed from blue to green upon interaction with the analyte. The developed probe was applied for the visualization of endogenous citrate in PC3 live cells. (C) 2017 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE SA AGGREGATION-INDUCED EMISSION INDICATOR-DISPLACEMENT ASSAY FLUORESCENT-PROBE SELECTIVE DETECTION LIVING CELLS ZINC COMPLEX SENSOR CHEMOSENSORS MITOCHONDRIA EFFICIENT Metal free bioimaging reagent for intracellular citrate in prostate cancer cells using aryl boronate derivative Article 10.1016/j.snb.2017.12.047 1 SENSORS AND ACTUATORS B-CHEMICAL, v.259, pp.90 - 96 SENSORS AND ACTUATORS B-CHEMICAL 259 90 96 scie scopus 000424877600012 2-s2.0-85038002175 Chemistry, Analytical Electrochemistry Instruments & Instrumentation Chemistry Electrochemistry Instruments & Instrumentation Article AGGREGATION-INDUCED EMISSION INDICATOR-DISPLACEMENT ASSAY FLUORESCENT-PROBE SELECTIVE DETECTION LIVING CELLS ZINC COMPLEX SENSOR CHEMOSENSORS MITOCHONDRIA EFFICIENT Citrate assay Boronate sensor Prostate cancer Fluorescence cell imaging