Kang, Suk Woo Kim, Kyung-A. Lee, Chung Hyun Yang, Sung Jae Kang, Tae Kyeom Jung, Je Hyeong Kim, Tae-Jin Oh, Sang-Rok Jung, Sang Hoon 2024-01-19T23:01:57Z 2024-01-19T23:01:57Z 2021-09-03 2018-04-06 0378-8741 https://pubs.kist.re.kr/handle/201004/121489 Ethnopharmacological relevance: In contrast to other leguminous plants generally used as food, Rhynchosia volubilis Loureiro, a small soybean with a black seed coat, has been used as a traditional oriental remedy for various human diseases in Eastern Asia. In this study, we demonstrated the protective effect of R. volubilis against dry eye disease. Aim of the study: We aimed to investigate whether a standardized ethanol extract of R. volubilis (EERV) can protect the cornea in a benzalkonium chloride (BAC)-induced mouse dry eye model. Materials and methods: Experimental dry eye was induced by the instillation of 0.2% BAC on mouse cornea. A standardized ethanol extract of R. volubilis (EERV) was orally administered following BAC treatment. The positive control group was treated with commercial eye drops. Fluorescein staining, tear break-up time (BUT), and hematoxylin and eosin staining were evaluated on the ocular surface. Squamous metaplasia and apoptosis in the corneal epithelial layer were detected by immunostaining. Furthermore, the protein expression of cytochrome c, BcI-2, and Bax was determined. Results: EERV treatment significantly improved fluorescein scoring, BUT, and smoothness in the cornea compared to the vehicle group. In addition, EERV inhibited squamous metaplasia and apoptosis in the cornea. The expression of cytochrome c and Bax was upregulated, while that of BcI-2 was downregulated in the vehicle group compared with that in the control group. However, EERV treatment inhibited the expression of cytochrome c and Bax, while that of Bcl-2 was improved. Conclusion: Standardized EERV could be a beneficial candidate for the treatment of dry eye disease. English ELSEVIER IRELAND LTD CORNEAL EPITHELIAL-CELLS PH-DEPENDENT INTERACTION OXIDATIVE STRESS BLACK SOYBEANS ANTIOXIDANT PROPERTIES IMPRESSION CYTOLOGY SQUAMOUS METAPLASIA TOTAL PHENOLICS CYTOCHROME-C SEED COAT A standardized extract of Rhynchosia volubilis Lour. exerts a protective effect on benzalkonium chloride-induced mouse dry eye model Article 10.1016/j.jep.2017.12.041 1 JOURNAL OF ETHNOPHARMACOLOGY, v.215, pp.91 - 100 JOURNAL OF ETHNOPHARMACOLOGY 215 91 100 scie scopus 000427213500009 2-s2.0-85039997024 Plant Sciences Chemistry, Medicinal Integrative & Complementary Medicine Pharmacology & Pharmacy Plant Sciences Pharmacology & Pharmacy Integrative & Complementary Medicine Article CORNEAL EPITHELIAL-CELLS PH-DEPENDENT INTERACTION OXIDATIVE STRESS BLACK SOYBEANS ANTIOXIDANT PROPERTIES IMPRESSION CYTOLOGY SQUAMOUS METAPLASIA TOTAL PHENOLICS CYTOCHROME-C SEED COAT Small black bean Dry eye disease Cornea Benzalkonium chloride Natural products 2.444: Ophthalmology 2.642: Traditional medicine Asia & Oceania 2.348: Inflammation