Son, Hyeoncheol Francis Lee, Sun-Mi Kim, Kyung-Jin 2024-01-19T23:03:14Z 2024-01-19T23:03:14Z 2021-09-03 2018-04 1017-7825 https://pubs.kist.re.kr/handle/201004/121559 Biofuel production using lignocellulosic biomass is gaining attention because it can be substituted for fossil fuels without competing with edible resources. However, because Saccharomyces cerevisiae does not have a D-xylose metabolic pathway, oxidoreductase or isomerase pathways must be introduced to utilize D-xylose from lignocellulosic biomass in S. cerevisiae. To elucidate the biochemical properties of xylose isomerase (XI) from Piromyces sp. E2 (PsXI), we determine its crystal structure in complex with substrate mimic glycerol. An amino-acid sequence comparison with other reported XIs and relative activity measurements using five kinds of divalent metal ions confirmed that PsXI belongs to class II XIs. Moreover kinetic analysis of PsXI was also performed using Mn2+, the preferred divalent metal ion for PsXI. In addition, the substrate-binding mode of PsXI could be predicted with the substrate mimic glycerol bound to the active site. These studies may provide structural information to enhance D-xylose utilization for biofuel production. English KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY RECOMBINANT SACCHAROMYCES-CEREVISIAE FUNCTIONAL EXPRESSION ETHANOL-PRODUCTION FERMENTATION STRAIN STATE Crystal Structure and Biochemical Characterization of Xylose Isomerase from Piromyces sp. Article 10.4014/jmb.1711.11026 1 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.28, no.4, pp.571 - 578 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 28 4 571 578 scie scopus kci ART002343065 000433223100010 2-s2.0-85046149583 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article RECOMBINANT SACCHAROMYCES-CEREVISIAE FUNCTIONAL EXPRESSION ETHANOL-PRODUCTION FERMENTATION STRAIN STATE Pentose metabolism D-xylose xylose isomerase Pyromyces sp E2