Ko, Jeong-Hyeon Nam, Dongwoo Um, Jae-Young Jung, Sang Hoon Ahn, Kwang Seok 2024-01-19T23:03:32Z 2024-01-19T23:03:32Z 2021-08-31 2018-04 0192-415X https://pubs.kist.re.kr/handle/201004/121575 Obesity is a serious and increasing health problem worldwide, and the inhibition of adipogenesis is considered to be a potential therapeutic target for it. Bergamottin (BGM), a component of grapefruit juice, has been reported to regulate lipolysis. However, the physiological role of BGM in obesity has not been evaluated so far. In the present study, we investigated the effects of BGM on obesity in 3T3-L1 cells and in mice fed a high-fat diet (HFD). BGM inhibited adipogenic differentiation of 3T3-L1 cells along with a significant decrease in the lipid content by downregulating the expression of two critical adipogenic factors, CCAAT enhancer-binding protein-alpha (C/EBP alpha) and peroxisome proliferator activated receptor-gamma (PPAR gamma). The expressions of target proteins such as adipocyte fatty acid-binding protein (aP2), adiponectin, and resistin were also decreased by BGM. It activated AMP-activated protein kinase (AMPK) by increasing phosphorylation of AMPK and the downstream target acetyl-CoA carboxylase (ACC), indicating that BGM exerted its antiadipogenic effect through AMPK activation. In the HFD-induced obese mouse model, BGM administration significantly reduced the weight and sizes of white adipose tissue as well as the weight gain of mice fed HFD. Moreover, UCP1 and PGC1 alpha expressions, well-known as brown adipocyte marker genes, were higher in the BGM-treated HFD mice than that in the HFD-induced obese mice. This study suggests that BGM suppress adipogenesis by AMPK activation in vitro and reduces body weight in vivo. English WORLD SCIENTIFIC PUBL CO PTE LTD ACTIVATED PROTEIN-KINASE STAT3 SIGNALING PATHWAY WHITE ADIPOSE-TISSUE ADIPOCYTE DIFFERENTIATION METABOLIC SYNDROME INSULIN SENSITIVITY ENERGY-BALANCE AMPK PATHWAY C/EBP-ALPHA PPAR-GAMMA Bergamottin Inhibits Adipogenesis in 3T3-L1 Cells and Weight Regulation in Diet-Induced Obese Mice Article 10.1142/S0192415X18500313 1 AMERICAN JOURNAL OF CHINESE MEDICINE, v.46, no.3, pp.601 - 615 AMERICAN JOURNAL OF CHINESE MEDICINE 46 3 601 615 scie scopus 000430831700007 2-s2.0-85044790013 Integrative & Complementary Medicine Medicine, General & Internal Integrative & Complementary Medicine General & Internal Medicine Article ACTIVATED PROTEIN-KINASE STAT3 SIGNALING PATHWAY WHITE ADIPOSE-TISSUE ADIPOCYTE DIFFERENTIATION METABOLIC SYNDROME INSULIN SENSITIVITY ENERGY-BALANCE AMPK PATHWAY C/EBP-ALPHA PPAR-GAMMA Bergamottin Adipogenesis Obesity AMPK High-Fat Diet