Hwang, Hongik Rhim, Hyewhon 2024-01-19T23:04:53Z 2024-01-19T23:04:53Z 2021-09-03 2018-03 1043-6618 https://pubs.kist.re.kr/handle/201004/121646 Post-(t)ranslational modifications (PTMs) covalently modify proteins and diversify protein functions. Along with protein phosphorylation, another common PTM is the addition of O-linked beta-N-acetylglucosamine (O-GlcNAc) to serine and/or threonine residues. O-GlcNAc modification is similar to phosphorylation in that it occurs to serine and threonine residues and cycles on and off with a similar time scale. However, a striking difference is that the addition and removal of the O-GlcNAc moiety on all substrates are mediated by the two enzymes regardless of proteins, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), respectively. O-GlcNAcylation can interact or potentially compete with phosphorylation on serine and threonine residues, and thus serves as an important molecular mechanism to modulate protein functions and activation. However, it has been challenging to address the role of O-GlcNAc modification in regulating protein functions at the molecular level due to the lack of convenient tools to determine the sites and degrees of O-GlcNAcylation. Studies in this field have only begun to expand significantly thanks to the recent advances in detection and manipulation methods such as quantitative proteomics and highly selective small-molecule inhibitors for OGT and OGA. Interestingly, multiple brain regions, especially hippocampus, express high levels of both OGT and OGA, and a number of neuron-specific proteins have been reported to undergo O-GlcNAcylation. This review aims to discuss the recent updates concerning the impacts of O-GlcNAc modification on neuronal functions at multiple levels ranging from intrinsic neuronal properties to synaptic plasticity and animal behaviors. (C) 2017 Elsevier Ltd. All rights reserved. English ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD LONG-TERM DEPRESSION SYNAPTIC PLASTICITY MODIFIED PROTEINS ALPHA-SYNUCLEIN LINKED GLCNAC NUTRIENT REGULATION GENE-EXPRESSION AMPA RECEPTORS AMYLOID-BETA TAU-PROTEIN Functional significance of O-GlcNAc modification in regulating neuronal properties Article 10.1016/j.phrs.2017.12.006 1 PHARMACOLOGICAL RESEARCH, v.129, pp.295 - 307 PHARMACOLOGICAL RESEARCH 129 295 307 scie scopus 000428102600028 2-s2.0-85037720160 Pharmacology & Pharmacy Pharmacology & Pharmacy Review LONG-TERM DEPRESSION SYNAPTIC PLASTICITY MODIFIED PROTEINS ALPHA-SYNUCLEIN LINKED GLCNAC NUTRIENT REGULATION GENE-EXPRESSION AMPA RECEPTORS AMYLOID-BETA TAU-PROTEIN O-GlcNAcylation Synaptic plasticity Post-translational modification O-GlcNAc transferase O-GlcNAcase Learning and memory