Kim, Kyung Min Nam, Yun-Sik Lee, Yeonhee Lee, Kang-Bong 2024-01-19T23:31:45Z 2024-01-19T23:31:45Z 2021-09-03 2018-02 2090-8865 https://pubs.kist.re.kr/handle/201004/121732 A highly sensitive and selective colorimetric assay for the detection of Hg2+ ions was developed using gold nanoparticles (AuNPs) conjugated with polyethyleneimine (PEI). The Hg2+ ion coordinates with PEI, decreasing the interparticle distance and inducing aggregation. Time-of-fight secondary ion mass spectrometry showed that the Hg2+ ion was bound to the nitrogen atoms of the PEI in a bidentate manner (N-Hg2+-N), which resulted in a significant color change from light red to violet due to aggregation. Using this PEI-AuNP probe, determination of Hg2+ ion can be achieved by the naked eye and spectrophotometric methods. Pronounced color change of the PEI-AuNPs in the presence of Hg2+ was optimized at pH 7.0, 50 degrees C, and 300mM. NaCl concentration. The absorption intensity ratio (A(700)/A(514)) was correlated with the Hg2+ concentration in the linear range of 0.003-5.0 mu M. The limits of detection were measured to be 1.72, 1.80, 2.00, and 1.95nM for tap water, pond water, tuna fish, and bovine serum, respectively. Owing to its facile and sensitive nature, this assay method for Hg2+ ions can be applied to the analysis of water and biological samples. English HINDAWI LTD MERCURY SPECIATION NON-AGGREGATION METAL-IONS THIMEROSAL TOXICITY STRATEGY GENE A Highly Sensitive and Selective Colorimetric Hg2+ Ion Probe Using Gold Nanoparticles Functionalized with Polyethyleneimine Article 10.1155/2018/1206913 1 JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY, v.2018 JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY 2018 scie scopus 000425405900001 2-s2.0-85042542428 Chemistry, Analytical Chemistry Article MERCURY SPECIATION NON-AGGREGATION METAL-IONS THIMEROSAL TOXICITY STRATEGY GENE