Kang, Kyungsu Peng, Lei Jung, Yu-Jin Kim, Joo Yeon Lee, Eun Ha Lee, Hee Ju Kim, Sang Min Sung, Sang Hyun Pan, Cheol-Ho Choi, Yongsoo 2024-01-19T23:31:53Z 2024-01-19T23:31:53Z 2021-09-03 2018-02 0141-5492 https://pubs.kist.re.kr/handle/201004/121740 To develop a high-throughput screening system to measure the conversion of testosterone to dihydrotestosterone (DHT) in cultured human prostate cancer cells using turbulent flow chromatography liquid chromatography-triple quadrupole mass spectrometry (TFC-LC-TQMS). After optimizing the cell reaction system, this method demonstrated a screening capability of 103 samples, including 78 single compounds and 25 extracts, in less than 12 h without manual sample preparation. Consequently, fucoxanthin, phenethyl caffeate, and Curcuma longa L. extract were validated as bioactive chemicals that inhibited DHT production in cultured DU145 cells. In addition, naringenin boosted DHT production in DU145 cells. The method can facilitate the discovery of bioactive chemicals that modulate the DHT production, and four phytochemicals are potential candidates of nutraceuticals to adjust DHT levels in male hormonal dysfunction. English SPRINGER 5-ALPHA-REDUCTASE INHIBITORS LNCAP High-throughput and direct measurement of androgen levels using turbulent flow chromatography liquid chromatography-triple quadrupole mass spectrometry (TFC-LC-TQMS) to discover chemicals that modulate dihydrotestosterone production in human prostate cancer cells Article 10.1007/s10529-017-2480-5 1 BIOTECHNOLOGY LETTERS, v.40, no.2, pp.263 - 270 BIOTECHNOLOGY LETTERS 40 2 263 270 scie scopus 000425115100005 2-s2.0-85034659305 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article 5-ALPHA-REDUCTASE INHIBITORS LNCAP 5 alpha-reductase inhibitor Dihydrotestosterone Fucoxanthin Naringenin Testosterone