Kim, Eun Sook Kim, Byeong-Soo Kim, Ki-Yeon Woo, Han-Min Lee, Sun-Mi Um, Youngsoon 2024-01-19T23:32:04Z 2024-01-19T23:32:04Z 2021-09-03 2018-02 0168-1656 https://pubs.kist.re.kr/handle/201004/121750 For cost-effective lignocellulosic biofuel/chemical production, consolidated bioprocessing (CBP)-enabling microorganisms utilizing cellulose as well as producing biofuel/chemical are required. A novel strain Paenibacillus sp. CAA11 isolated from sediment was found to be not only as a cellulose degrader under both aerobic and strict anaerobic conditions but also as a producer of cellulosic biofuel/chemicals. Paenibacillus sp. CAA11 secreted cellulolytic enzymes by its own secretion system and produced ethanol as well as short-chain organic acids (formic acid, acetic acid, lactic acid) from cellulose. Cellulolytic activity of the strain was significantly enhanced by expressing a heterologous endoglucanase 168Cel5 from Bacillus subtilis under both aerobic and anaerobic conditions. The strain harboring the 168cel5 gene revealed 2-fold bigger halo zone on Congo-red plate and 1.75-fold more aerobic cellulose utilization in liquid medium compared with the negative control. Notably, under anaerobic conditions, the recombinant strain expressing 168Cel5 consumed 1.83-fold more cellulose (5.10 g/L) and produced 5-fold more ethanol (0.65 g/L) along with 5-fold more total acids (1.6 g/L) compared with the control, resulting 2.73-fold higher yields. This result demonstrates the potential of Paenibacillus sp. CAA11 as a suitable aerobic and anaerobic CBP-enabling microbe with cellulolytic production of ethanol and short-chain organic acids. English Elsevier BV Aerobic and anaerobic cellulose utilization by Paenibacillus sp CAA11 and enhancement of its cellulolytic ability by expressing a heterologous endoglucanase Article 10.1016/j.jbiotec.2018.01.007 1 Journal of Biotechnology, v.268, pp.21 - 27 Journal of Biotechnology 268 21 27 N scie scopus 000425715100004 2-s2.0-85041317542 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article CURDLANOLYTICUS STRAIN B-6 CLOSTRIDIUM-CELLULOLYTICUM ETHANOL-PRODUCTION ZYMOMONAS-MOBILIS GENE SACCHARIFICATION SEQUENCE ENZYMES FERMENTATION DEGRADATION Cellulose Paenibacillus Consolidated bioprocessing Endoglucanase