Han, Heetak Lee, Jung Seung Kim, Hyunchul Shin, Sera Lee, Jaehong Kim, Jongchan Hou, Xu Cho, Seung-Woo Seo, Jungmok Lee, Taeyoon 2024-01-19T23:32:07Z 2024-01-19T23:32:07Z 2021-09-03 2018-02 1936-0851 https://pubs.kist.re.kr/handle/201004/121753 Herein, a droplet manipulation system with a superamphiphobic (SPO)-superamphiphilic (SPI) patterned polydimethylsiloxane (PDMS) substrate is developed for a multiplex bioassay from single-droplet samples. The SPO substrate is fabricated by sequential spraying of adhesive and fluorinated silica nanoparticles onto a PDMS substrate. It is subsequently subjected to oxygen plasma with a patterned mask to form SPI patterns. The SPO layer exhibits extreme liquid repellency with a high contact angle (>150 degrees) toward low surface tension and viscous biofluidic droplets (e.g., ethylene glycol, blood, dimethyl sulfoxide, and alginate hydrogel). In contrast, the SPI exhibits liquid adhesion with a near zero contact angle. Using the droplet manipulation system, various liquid droplets can be precisely manipulated and dispensed onto the predefined SPI patterns on the SPO PDMS substrate. This system enables a multiplex colorimetric bioassay, capable of detecting multiple analytes, including glucose, uric acid, and lactate, from a single sample droplet. In addition, the detection of glucose concentrations in a plasma droplet of diabetic and healthy mice are performed to demonstrate the feasibility of the proposed system for efficient clinical diagnostic applications. English AMER CHEMICAL SOC BIO-BARCODE ASSAY COLORIMETRIC BIOASSAY SURFACE-TENSION ADHESION PLATFORM GLUCOSE FABRICATION PROTEIN DEVICE CHIP Single-Droplet Multiplex Bioassay on a Robust and Stretchable Extreme Wetting Substrate through Vacuum-Based Droplet Manipulation Article 10.1021/acsnano.7b05826 1 ACS NANO, v.12, no.2, pp.932 - 941 ACS NANO 12 2 932 941 scie scopus 000426615600004 2-s2.0-85042726251 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Article BIO-BARCODE ASSAY COLORIMETRIC BIOASSAY SURFACE-TENSION ADHESION PLATFORM GLUCOSE FABRICATION PROTEIN DEVICE CHIP superamphiphobic bioassay droplet manipulation colorimetric analysis biomimetics