Lee, Ga-Yeon Park, Jun-Hee Chang, Young Wook Cho, Sungbo Kang, Min Jung Pyun, Jae-Chul 2024-01-19T23:33:51Z 2024-01-19T23:33:51Z 2021-09-03 2018-01 2379-3694 https://pubs.kist.re.kr/handle/201004/121845 In this work, the chronoamperometry-based redox cycling of 3,3',5,5'-tetramethylbenzidine (TMB) was performed by using interdigitated electrode (IDE). The signal was obtained from two sequential chronoamperometric profiles: (1) with the generator at the oxidative potential of TMB and the collector at the reductive potential of TMB, and (2) with the generator at the reductive potential of TMB and the collector at the oxidative potential of TMB. The chronoamperometry-based redox cycling (dual mode) showed a sensitivity of 1.49 mu A/OD, and the redox cycling efficiency was estimated to be 94% (n = 10). The sensitivities of conventional redox cycling with the same interdigitated electrode and chronoamperometry using a single working electrode (single mode) were estimated to be 0.67 mu A/OD and 0.18 mu A/OD, respectively. These results showed that the chronoamperometry-based redox cycling (dual mode) could be more effectively used to quantify the oxidized TMB than other amperometric methods. The chronoamperometry-based redox cycling (dual mode) was applied to immunoassays using a commercial ELISA kit for medical diagnosis of the human hepatitis B virus surface antigen (hHBsAg). Finally, the chronoamperometry-based redox cycling (dual mode) provided more than a 10-fold higher sensitivity than conventional chronoamperometry using a single working electrode (single mode) when applied to a commercial ELISA kit for medical diagnosis of hHBsAg. English AMER CHEMICAL SOC HEPATITIS-B-VIRUS AUTODISPLAYED Z-DOMAINS INTERDIGITATED ELECTRODES NANOELECTRODE ARRAYS ESCHERICHIA-COLI SPR BIOSENSOR SIMULATION NANOWIRES DOPAMINE ANTIGEN Chronoamperometry-Based Redox Cycling for Application to Immunoassays Article 10.1021/acssensors.7b00681 1 ACS Sensors, v.3, no.1, pp.106 - 112 ACS Sensors 3 1 106 112 scie scopus 000423782600014 2-s2.0-85041235319 Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Chemistry Science & Technology - Other Topics Article HEPATITIS-B-VIRUS AUTODISPLAYED Z-DOMAINS INTERDIGITATED ELECTRODES NANOELECTRODE ARRAYS ESCHERICHIA-COLI SPR BIOSENSOR SIMULATION NANOWIRES DOPAMINE ANTIGEN redox cycling chronoamperometry ELISA kit 3,3 &apos ,5,5 &apos -tetramethylbenzidine hepatitis B virus