Kim, In Gul Gil, Chang-Hyun Seo, Joseph Park, Soon-Jung Ramesh Subbiah Jung, Taek-Hee Kim, Jong Soo Jeong, Young-Hoon Chung, Hyung-Min Lee, Jong Ho Lee, Man Ryul Moon, Sung-Hwan Park, Kwideok 2024-01-19T23:33:52Z 2024-01-19T23:33:52Z 2021-09-03 2018-01 0142-9612 https://pubs.kist.re.kr/handle/201004/121846 Cell-derived matrices (CDM) are becoming an attractive alternative to conventional biological scaffolding platforms due to its unique ability to closely recapitulate a native extracellular matrix (ECM) de novo. Although cell-substrate interactions are recognized to be principal in regulating stem cell behavior, very few studies have documented the acclimation of human pluripotent stem cells (hPSCs) on pristine and altered cell-derived matrices. Here, we investigate crosslink-induced mechanotransduction of hPSCs cultivated on decellularized fibroblast-derived matrices (FDM) to explore cell, adhesion, growth, migration, and pluripotency in various biological landscapes. The results showed either substrate-mediated induction or inhibition of the Epithelial-Mesenchymal-Transition (EMT) program, strongly suggesting that FDM stiffness can be a dominant factor in mediating hPSC plasticity. We further propose an optimal FDM substratum intended for long-term hPSC cultivation in a feeder-free niche-like microenvironment. This study carries significant implications for hPSC cultivation and encourages more in-depth studies towards the fundamentals of hPSC-CDM interactions. (C) 2017 Elsevier Ltd. All rights reserved. English Pergamon Press Ltd. Mechanotransduction of human pluripotent stem cells cultivated on tunable cell-derived extracellular matrix Article 10.1016/j.biomaterials.2017.10.016 1 Biomaterials, v.150, pp.100 - 111 Biomaterials 150 100 111 N scie scopus 000415781900008 2-s2.0-85031111577 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article EPITHELIAL-MESENCHYMAL TRANSITION SELF-RENEWAL CANCER-CELLS DIFFERENTIATION ADHESION GROWTH FATE GENE