Kwon, Sung-Pil Jeon, Sangmin Lee, Sung-Hoon Yoon, Hong Yeol Ryu, Ju Hee Choi, Dayil Kim, Jeong-Yeon Kim, Jiwon Park, Jae Hyung Kim, Dong-Eog Kwon, Ick Chan Kim, Kwangmeyung Ahn, Cheol-Hee 2024-01-19T23:33:55Z 2024-01-19T23:33:55Z 2021-09-03 2018-01 0142-9612 https://pubs.kist.re.kr/handle/201004/121849 Thrombosis is an important pathophysiologic phenomenon in various cardiovascular diseases, which can lead to oxygen deprivation and infarction of tissues by generation of a thrombus. Thus, direct thrombus imaging can provide beneficial in diagnosis and therapy of thrombosis. Herein, we developed thrombin-activatable fluorescent peptide (TAP) incorporated silica-coated gold nanoparticles (TAP-SiO2@AuNPs) for direct imaging of thrombus by dual near-infrared fluorescence (NIRF) and micro-computed tomography (micro-CT) imaging, wherein TAP molecules were used as targeted thrombin-activatable peptide probes for thrombin-specific NIRF imaging. The freshly prepared TAP-SiO2@AuNPs had an average diameter of 39.8 +/- 2.55 nm and they showed the quenched NIRF signal in aqueous condition, due to the excellent quenching effect of TAP molecules on the silica-gold nanoparticle surface. However, 30.31-fold higher NIRF intensity was rapidly recovered in the presence of thrombin in vitro, due to the thrombin specific cleavage of quenched TAP molecules on the gold particle surface. Furthermore, TAP-SiO2@AuNPs were successfully accumulated in thrombus by their particle size-dependent capturing property, and they presented a potential X-ray absorption property in a dose-dependent manner. Finally, thrombotic lesion was clearly distinguished from peripheral tissues by dual NIRF/micro-CT imaging after intravenous injection of TAP-SiO2@AuNPs in the in situ thrombotic mouse model, simultaneously. This study showed that thrombin-activatable fluorescent peptide incorporated silica-coated gold nano particles can be potentially used as a dual imaging probe for direct thrombus imaging and therapy in clinical applications. (C) 2017 Elsevier Ltd. All rights reserved. English Pergamon Press Ltd. Thrombin-activatable fluorescent peptide incorporated gold nanoparticles for dual optical/computed tomography thrombus imaging Article 10.1016/j.biomaterials.2017.10.017 1 Biomaterials, v.150, pp.125 - 136 Biomaterials 150 125 136 N scie scopus 000415781900010 2-s2.0-85031116713 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article CEREBRAL VENOUS THROMBOSIS COMPUTED-TOMOGRAPHY SILICA NANOPARTICLES RISK-FACTORS FIBRIN CT THROMBOEMBOLISM QUANTIFICATION POLYMERIZATION MECHANISMS Silica-coated gold nanoparticle Thrombin-activatable fluorescent probe Thrombus imaging Dual optical/CT imaging