Hong, Sungeun Kwon, Jaeyoung Nguyen Tuan Hiep Sim, Su Jin Kim, Nahyun Kim, Kyeong Ho Lee, Dongho Mar, Woongchon 2024-01-19T23:34:17Z 2024-01-19T23:34:17Z 2021-09-03 2018-01 1756-4646 https://pubs.kist.re.kr/handle/201004/121869 This study evaluated the neuroprotective effects 50% EtOH extract of Maclura tricuspidata fruits (FME50) and isolated compounds, cudraisoflavone I (CFI), and cudraisoflavone H (CFH), on ischemic damage in in vitro model and in vivo model of cerebral ischemia. FME50, CFI, and CFH inhibited OGD/R + glucose-induced neuronal cell death, ROS generation, and Nox4 expression via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a in SH-SY5Y cells. Also, FME50 suppressed OGD/R + glucose-induced activation of ASK1-JNK1/p38 MAPK signal cascade. Furthermore, FME50 significantly reduced the MCAO/R-induced brain infarct, Nox4 expression via induction of Nox4-targeting three miRNAs. Additionally, FME50 suppressed MCAO/R-induced MAPK signal pathway. These results demonstrate that FME50, CFI, and CFH exert neuroprotective effects via Nox4 inhibition by the induction of Nox4-targeting miRNAs and inhibition of MAPK signal cascade, suggesting that they might be possible candidates for the treatment of cerebral ischemia. English ELSEVIER ENDOPLASMIC-RETICULUM STRESS CUDRANIA-TRICUSPIDATA FOCAL ISCHEMIA NADPH OXIDASES NOX FAMILY EXPRESSION APOPTOSIS BRAIN ACTIVATION INHIBITION The isoflavones and extracts from Maclura tricuspidata fruit protect against neuronal cell death in ischemic injury via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a Article 10.1016/j.jff.2017.12.011 1 JOURNAL OF FUNCTIONAL FOODS, v.40, pp.785 - 797 JOURNAL OF FUNCTIONAL FOODS 40 785 797 scie scopus 000428005500086 2-s2.0-85037976304 Food Science & Technology Nutrition & Dietetics Food Science & Technology Nutrition & Dietetics Article ENDOPLASMIC-RETICULUM STRESS CUDRANIA-TRICUSPIDATA FOCAL ISCHEMIA NADPH OXIDASES NOX FAMILY EXPRESSION APOPTOSIS BRAIN ACTIVATION INHIBITION Maclura tricuspidata MiroRNAs Oxygen-glucose deprivation/reoxygenation plus glucose Middle cerebral artery occlusion/reperfusion NADPH oxidase MAPkinase