Park, Yae Eun Yeom, Jeonghun Kim, YoungSoo Lee, Hye Jin Han, Ki-Cheol Lee, Seung-Taek Lee, Cheolju Lee, Ji Eun 2024-01-19T23:34:23Z 2024-01-19T23:34:23Z 2021-09-03 2018-01 1615-9853 https://pubs.kist.re.kr/handle/201004/121874 Glioblastoma, also known as glioblastoma multiforme (GBM), is the most malignant type of brain cancer and has poor prognosis with a median survival of less than one year. While the structural changes of tumor cell surface carbohydrates are known to be associated with invasive behavior of tumor cells, the cell surface glycoproteins to differentiate the low-and high-grade glioma cells can be potential diagnostic markers and therapeutic targets for GBMs. In the present study, lectin arrays consisting of eight lectins were employed to explore cell surface carbohydrate expression patterns on low-grade oligodendroglioma cells (Hs683) and GBM cells (T98G). Griffonia simplicifolia I (GS I) was found to selectively bind to T98G cells and not to Hs683 cells. For identification of the glioblastoma-specific cell surface markers, the glycoproteins from each cell type were captured by a GS I lectin column and analyzed by LC-MS/MS. The identified proteins from the two cell types were quantified using label-free quantitative analysis based on spectral counting. Of cell surface glycoproteins showing significant increases in T98G cells, five proteins were selected for verification of both protein and glycosylation level changes using Western blot and GS I lectin-based immunosorbent assay. English WILEY SPECTRAL ABUNDANCE FACTORS ALEURIA-AURANTIA LECTIN ADHESION MOLECULE SHOTGUN PROTEOMICS SURFACE GLYCOSYLATION BIOMARKER DISCOVERY CARCINOMA-CELLS LUNG-CANCER EXPRESSION INVASION Identification of Plasma Membrane Glycoproteins Specific to Human Glioblastoma Multiforme Cells Using Lectin Arrays and LC-MS/MS Article 10.1002/pmic.201700302 1 PROTEOMICS, v.18, no.1 PROTEOMICS 18 1 scie scopus 000419942500004 2-s2.0-85040744104 Biochemical Research Methods Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article SPECTRAL ABUNDANCE FACTORS ALEURIA-AURANTIA LECTIN ADHESION MOLECULE SHOTGUN PROTEOMICS SURFACE GLYCOSYLATION BIOMARKER DISCOVERY CARCINOMA-CELLS LUNG-CANCER EXPRESSION INVASION cell surface glycoprotein glioblastoma multiforme (GBM) label-free quantitative MS lectin arrays