Park, Jooho Choi, Jeong Uk Kim, Kwangmeyung Byun, Youngro 2024-01-20T00:01:42Z 2024-01-20T00:01:42Z 2021-09-03 2017-12 0142-9612 https://pubs.kist.re.kr/handle/201004/121977 The development of highly funtional and orally available nanoparticles is the ultimate goal in nano particle delivery. Various functional nanoparticles have been studied to that end but there has yet to be an oral nanoparticle that can be successfully applied. Here, we describe for the first time a novel bile acid conjugated nanoparticle that can be selectively absorbed by bile acid transporters in the small intestine. The bile acid conjugate nanoparticles that were first treated with enterocytes were successfully attached to the cell surface and then internalized inside the cells. We show that bile acid based interaction between a nanoparticle and its transporter induces its endocytosis and cellular uptake. This feature of cellular activity, described here for the first time, could be well utilized in the uptake of nanoparticles or macromolecules inside epithelial cells for oral delivery. In animal studies, bile acid conjugated self assembling nanocomplexes successfully interacted with bile acid transporters in the ileum and were subsequently taken up into the epithelial cells. Considering the importance of orally deliverable nano particles, this nanotechnology using bile acid conjugation and transporter mediated endocytosis could be a crucial method for the successful application of various nanoparticles. (C) 2017 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD MOLECULAR-WEIGHT HEPARIN DRUG-DELIVERY-SYSTEMS OF-THE-ART DEOXYCHOLIC-ACID GASTROINTESTINAL MUCUS TARGETED NANOPARTICLES ANTICOAGULANT EFFICACY GENE DELIVERY THERAPY INSULIN Bile acid transporter mediated endocytosis of oral bile acid conjugated nanocomplex Article 10.1016/j.biomaterials.2017.09.022 1 BIOMATERIALS, v.147, pp.145 - 154 BIOMATERIALS 147 145 154 scie scopus 000413609700011 2-s2.0-85029805165 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article MOLECULAR-WEIGHT HEPARIN DRUG-DELIVERY-SYSTEMS OF-THE-ART DEOXYCHOLIC-ACID GASTROINTESTINAL MUCUS TARGETED NANOPARTICLES ANTICOAGULANT EFFICACY GENE DELIVERY THERAPY INSULIN Oral nanoparticle Bile acid Heparin Protamine Endocytosis Drug carrier