Rehman, Gauhar Hamayun, Muhammad Iqbal, Amjad Khan, Sumera Afzal Khan, Hamayoon Shehzad, Adeeb Khan, Abdul Latif Hussain, Anwar Kim, Ho-Youn Ahmad, Jamshaid Ahmad, Ayaz Ali, Abid Lee, In-Jung 2024-01-20T00:02:47Z 2024-01-20T00:02:47Z 2021-09-03 2017-11-28 1663-9812 https://pubs.kist.re.kr/handle/201004/122036 Ethnomedicinal knowledge of plant-derived bioactives could help us in discovering new therapeutic compounds of great potential. Certainly, dandelion has been used in traditional ethno-medicinal systems (i. e., Chinese, Arabian, Indian, and Native American) to treat different types of cancer. Though, dandelion is highly vigorous, but the potential mode of action is still unclear. In the current study, the antiproliferative activity of methanolic extracts of dandelion root (MEDr) on cell viability of HepG2, MCF7, HCT116, and normal Hs27 was investigated. It was observed that MEDr (500 m g/mL) drastically decreased the growth of HepG2 cell line, while the effect on MCF7 and HCT116 cell lines was less pronounced and no effect has been observed in Hs27 cell lines. The MEDr also enhanced the phosphorylation level of AMPK of HepG2 cells, which considered crucial in cancer treatment and other metabolic diseases. The AMPK activation by MEDr noticed in the current study has never been reported previously. The results regarding the number of apoptotic cells (HepG2 cells) were in line with the cell viability test. The current observations clearly demonstrated the potency of MEDr against liver cancer with validation that dandelion could control AMPK and thus cancer in the treated cell lines. English FRONTIERS MEDIA SA TARAXACUM-OFFICINALE BREAST-CANCER APOPTOSIS PROLIFERATION GROWTH FAMILY PLANT DEATH BAX Effect of Methanolic Extract of Dandelion Roots on Cancer Cell Lines and AMP-Activated Protein Kinase Pathway Article 10.3389/fphar.2017.00875 1 FRONTIERS IN PHARMACOLOGY, v.8 FRONTIERS IN PHARMACOLOGY 8 scie scopus 000416318200001 2-s2.0-85035347887 Pharmacology & Pharmacy Pharmacology & Pharmacy Article TARAXACUM-OFFICINALE BREAST-CANCER APOPTOSIS PROLIFERATION GROWTH FAMILY PLANT DEATH BAX cancer AMPK cytotoxicity traditional medicine dandelion