You, Dong Gil Yoon, Hong Yeol Jeon, Sangmin Um, Wooram Son, Sejin Park, Jae Hyung Kwon, Ick Chan Kim, Kwangmeyung 2024-01-20T00:04:00Z 2024-01-20T00:04:00Z 2021-09-04 2017-11 2196-5404 https://pubs.kist.re.kr/handle/201004/122099 Recently, ultrasound (US)-based drug delivery strategies have received attention to improve enhanced permeation and retention (EPR) effect-based passive targeting efficiency of nanoparticles in vitro and in vivo conditions. Among the US treatment techniques, pulsed-high intensity focused ultrasound (pHIFU) have specialized for improving tissue penetration of various macromolecules and nanoparticles without irreversible tissue damages. In this study, we have demonstrated that pHIFU could be utilized to improve tissue penetration of fluorescent dye-labeled glycol chitosan nanoparticles (FCNPs) in femoral tissue of mice. pHIFU could improve blood flow of the targeted-blood vessel in femoral tissue. In addition, tissue penetration of FCNPs was specifically increased 5.7-, 8- and 9.3-folds than that of non-treated (0W pHIFU) femoral tissue, when the femoral tissue was treated with 10, 20 and 50W of pHIFU, respectively. However, tissue penetration of FCNPs was significantly reduced after 3 h post-pHIFU treatment (50W). Because overdose (50W) of pHIFU led to irreversible tissue damages, including the edema and chapped red blood cells. These overall results support that pHIFU treatment can enhance the extravasation and tissue penetration of FCNPs as well as induce irreversible tissue damages. We expect that our results can provide advantages to optimize pHIFU-mediated delivery strategy of nanoparticles for further clinical applications. English Springer | Korea Nano Technology Research Society Deep tissue penetration of nanoparticles using pulsed-high intensity focused ultrasound Article 10.1186/s40580-017-0124-z 1 Nano Convergence, v.4, no.1 Nano Convergence 4 1 Y scopus 000455348100030 2-s2.0-85071453014 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Science & Technology - Other Topics Materials Science Physics Article Pulsed-high intensity focused ultrasound Tissue penetration Nanoparticle Drug delivery