Kim, Go-Woon Nam, Gi-Hoon Kim, In-San Park, Seung-Yoon 2024-01-20T00:04:43Z 2024-01-20T00:04:43Z 2021-09-03 2017-11 1742-464X https://pubs.kist.re.kr/handle/201004/122141 Xk-related protein 8 (Xkr8) is a scramblase and responsible for phosphatidylserine (PS) exposure on the cell surface in a caspase-dependent manner. Although PS exposure is found to be important for myotube formation during myoblast differentiation, the role of Xkr8 during myogenesis has not been elucidated. Here we show that Xkr8 contributes to myoblast differentiation. Xkr8 overexpression induced the formation of large myotubes during early differentiation, but this phenotype was not related to caspase-dependent cleavage of Xkr8. Furthermore, forced Xkr8 expression accelerated myoblast differentiation and conferred cell-death resistance after the induction of differentiation. Consistent with these results, Xkr8-knocked-down myoblasts exhibited impaired differentiation and more apoptotic cells during differentiation, implying the involvements of Xkr8 in the survival and proliferation of myoblasts. Taken together, the study shows Xkr8 influences myogenesis by acting as a positive regulator of terminal differentiation and myoblast survival. English WILEY APOPTOTIC PHOSPHATIDYLSERINE EXPOSURE SKELETAL-MUSCLE DIFFERENTIATION FLOW-CYTOMETRIC DETECTION MCLEOD-SYNDROME MYOCYTE DIFFERENTIATION ANNEXIN-V FUSION EXPRESSION KELL CELLS Xk-related protein 8 regulates myoblast differentiation and survival Article 10.1111/febs.14261 1 FEBS JOURNAL, v.284, no.21, pp.3575 - 3588 FEBS JOURNAL 284 21 3575 3588 scie scopus 000414918100004 2-s2.0-85033362497 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article APOPTOTIC PHOSPHATIDYLSERINE EXPOSURE SKELETAL-MUSCLE DIFFERENTIATION FLOW-CYTOMETRIC DETECTION MCLEOD-SYNDROME MYOCYTE DIFFERENTIATION ANNEXIN-V FUSION EXPRESSION KELL CELLS apoptosis differentiation myoblasts scramblase Xk-related protein 8