Kim, Min Hyung Lee, Jeongjick Nam, Kihwan Park, In Soo Son, Myeonggu Ko, Hyunchul Lee, Sangyoup Yoon, Dae Sung Chang, Woo-Jin Lee, Sei Young Yoon, Young Ro Lee, Sang Woo 2024-01-20T00:31:22Z 2024-01-20T00:31:22Z 2021-09-05 2017-10 1424-8220 https://pubs.kist.re.kr/handle/201004/122210 We reported an automated dielectrophoretic (DEP) tweezers-based force spectroscopy system to examine intermolecular weak binding interactions, which consists of three components: (1) interdigitated electrodes and micro-sized polystyrene particles used as DEP tweezers and probes inside a microfluidic device, along with an arbitrary function generator connected to a high voltage amplifier; (2) microscopy hooked up to a high-speed charge coupled device (CCD) camera with an image acquisition device; and (3) a computer aid control system based on the LabVIEW program. Using this automated system, we verified the measurement reliability by measuring intermolecular weak binding interactions, such as hydrogen bonds and Van der Waals interactions. In addition, we also observed the linearity of the force loading rates, which is applied to the probes by the DEP tweezers, by varying the number of voltage increment steps and thus affecting the linearity of the force loading rates. This system provides a simple and low-cost platform to investigate intermolecular weak binding interactions. English MDPI SCANNING PROBE MICROSCOPY PROTEIN-BOUND PARTICLES SINGLE-MOLECULE OPTICAL TWEEZERS ELUTION CLAMP Automated Dielectrophoretic Tweezers-Based Force Spectroscopy System in a Microfluidic Device Article 10.3390/s17102272 1 SENSORS, v.17, no.10 SENSORS 17 10 scie scopus 000414931500114 2-s2.0-85031278663 Chemistry, Analytical Engineering, Electrical & Electronic Instruments & Instrumentation Chemistry Engineering Instruments & Instrumentation Article SCANNING PROBE MICROSCOPY PROTEIN-BOUND PARTICLES SINGLE-MOLECULE OPTICAL TWEEZERS ELUTION CLAMP dielectrophoresis force spectroscopy force loading rate intermolecular weak binding interactions