Shon, Soo-Min Jang, Hee Jeong Schellingerhout, Dawid Kim, Jeong-Yeon Ryu, Wi-Sun Lee, Su-Kyoung Kim, Jiwon Park, Jin-Yong Oh, Ji Hye Kang, Jeong Wook Je, Kang-Hoon Park, Jung E. Kim, Kwangmeyung Kwon, Ick Chan Lee, Juneyoung Nahrendorf, Matthias Park, Jong-Ho Kim, Dong-Eog 2024-01-20T00:31:31Z 2024-01-20T00:31:31Z 2021-09-05 2017-10 1346-9843 https://pubs.kist.re.kr/handle/201004/122218 Background: The aim of this study is to identify the principal circulating factors that modulate atheromatous matrix metalloproteinase (MMP) activity in response to diet and exercise. Methods and Results: Apolipoprotein-E knock-out (ApoE(-/-)) mice (n= 56) with pre-existing plaque, fed either a Western diet (WD) or normal diet (ND), underwent either 10 weeks of treadmill exercise or had no treatment. Atheromatous MMP activity was visualized using molecular imaging with a MMP-2/9 activatable near-infrared fluorescent (NIRF) probe. Exercise did not significantly reduce body weight, visceral fat, and plaque size in either WD-fed animals or ND-fed animals. However, atheromatous MMP-activity was different; ND animals that did or did not exercise had similarly low MMP activities, WD animals that did not exercise had high MMP activity, and WD animals that did exercise had reduced levels of MMP activity, close to the levels of ND animals. Factor analysis and path analysis showed that soluble vascular cell adhesion molecule (sVCAM)-1 was directly positively correlated to atheromatous MMP activity. Adiponectin was indirectly negatively related to atheromatous MMP activity by way of sVCAM-1. Resistin was indirectly positively related to atheromatous MMP activity by way of sVCAM-1. Visceral fat amount was indirectly positively associated with atheromatous MMP activity, by way of adiponectin reduction and resistin elevation. MMP-2/9 imaging of additional mice (n= 18) supported the diet/exercise-related anti-atherosclerotic roles for sVCAM-1. Conclusions: Diet and exercise affect atheromatous MMP activity by modulating the systemic inflammatory milieu, with sVCAM-1, resistin, and adiponectin closely interacting with each other and with visceral fat. English JAPANESE CIRCULATION SOC E-DEFICIENT MICE IN-VIVO ATHEROSCLEROTIC LESIONS COMPUTED-TOMOGRAPHY ADHESION MOLECULES ENDOTHELIAL-CELLS VISCERAL OBESITY ADIPONECTIN INFLAMMATION MACROPHAGES Cytokine Response to Diet and Exercise Affects Atheromatous Matrix Metalloproteinase-2/9 Activity in Mice Article 10.1253/circj.CJ-16-1196 1 CIRCULATION JOURNAL, v.81, no.10, pp.1528 - + CIRCULATION JOURNAL 81 10 1528 + scie scopus 000411451200022 2-s2.0-85030128495 Cardiac & Cardiovascular Systems Cardiovascular System & Cardiology Article E-DEFICIENT MICE IN-VIVO ATHEROSCLEROTIC LESIONS COMPUTED-TOMOGRAPHY ADHESION MOLECULES ENDOTHELIAL-CELLS VISCERAL OBESITY ADIPONECTIN INFLAMMATION MACROPHAGES Atheroma Atherosclerosis Cytokine Matrix metalloproteinase Molecular imaging