Huda, Md Nazmul Erdene-Ochir, Erdenedolgor Pan, Cheol-Ho 2024-01-20T00:32:08Z 2024-01-20T00:32:08Z 2021-09-03 2017-10 1940-087X https://pubs.kist.re.kr/handle/201004/122252 The microtubule-associated protein tau is a neuronal protein that localizes mostly in axons. Generally tau is essential for normal neuronal functioning because it is involved in microtubule assembly and stabilization. Besides neurons, tau is expressed in human breast, prostate, gastric, colorectal, and pancreatic cancers where it shows nearly similar structure and exerts similar functions as the neuronal tau. The amount of tau and its phosphorylation can change its function as a stabilizer of microtubules, and lead to the development of paired helical filaments in different neurodegenerative disorders, such as Alzheimer's disease. Determining the phosphorylation state of tau and its microtubule-binding characteristics is important. In addition, examining the intracellular localization of tau is important in different diseases. This manuscript details standard protocols for measuring tau phosphorylation and tau binding to microtubules in colorectal cancer cells with or without curcumin and LiCl treatment. These treatments can be used to stop cancer cell proliferation and development. Intracellular localization of tau is examined by using immunohistochemistry and confocal microscopy while using low amounts of antibodies. These assays can be used repetitively for screening compounds that affect tau hyperphosphorylation or microtubule binding. Novel therapeutics used for different tauopathies or related anticancer agents can potentially be characterized using these protocols. English JOURNAL OF VISUALIZED EXPERIMENTS Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells Article 10.3791/55932 1 JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v.2017, no.128 JOVE-JOURNAL OF VISUALIZED EXPERIMENTS 2017 128 N scie scopus 000415371500018 2-s2.0-85031009658 Multidisciplinary Sciences Science & Technology - Other Topics Article PAIRED HELICAL FILAMENTS NEUROFIBRILLARY TANGLES ALZHEIMERS-DISEASE CURCUMIN EXPRESSION INHIBITION ISOFORMS PURIFICATION MODEL Alzheimer&apos s disease tauopathy phosphorylation microtubules curcumin lithium chloride confocal microscopy