Jeun, Minhong Park, Sungwook Kim, Yongdeok Choi, Jaewon Song, Sang Hoon Jeong, In Gab Kim, Choung-Soo Lee, Kwan Hyi 2024-01-20T00:33:06Z 2024-01-20T00:33:06Z 2021-09-05 2017-09-06 2192-2640 https://pubs.kist.re.kr/handle/201004/122301 A noninvasive quantitative assay that is capable of identifying prostate cancer biomarkers in untreated urine is an attractive diagnosis tool, but this method is subject to various obstacles. Difficulties presented by untreated urine include varying salt concentrations, and pH levels that may be different even though they are from the same patient. Untreated urine also presents interference from other biomolecules and possesses a fewer number of cancer biomarkers than can be found in serum. As a result, urine preconditioning processes and digital rectal examination (DRE) to increase biomarker secretion are mandatory in current urine assays. To address these challenges, an ion-responsive urine sensor (IRUS) that measures differential electrical signals is proposed as a self-normalized detection method. The proposed IRUS is based on a FET biosensor with a disposable sensing gate and has the capability to detect the prostate cancer antigen ANXA3 in untreated patient urine. The IRUS can detect ANXA3 at <1 fg mL(-1) with high reliability. In addition, it is found that ANXA3 levels in urine show clinically significant correlation with real tumor volumes. This paper provides a guideline in developing a clinically ready accurate noninvasive platform, which is capable of predicting prostate cancer using untreated urine without DRE. English WILEY FIELD-EFFECT TRANSISTOR ANNEXIN A3 ELECTROCHEMICAL SENSOR SERUM ANTIGEN PARTICLES VOLUME PSA Self-Normalized Detection of ANXA3 from Untreated Urine of Prostate Cancer Patients without Digital Rectal Examination Article 10.1002/adhm.201700449 1 ADVANCED HEALTHCARE MATERIALS, v.6, no.17 ADVANCED HEALTHCARE MATERIALS 6 17 scie scopus 000409410500004 2-s2.0-85023174152 Engineering, Biomedical Nanoscience & Nanotechnology Materials Science, Biomaterials Engineering Science & Technology - Other Topics Materials Science Article FIELD-EFFECT TRANSISTOR ANNEXIN A3 ELECTROCHEMICAL SENSOR SERUM ANTIGEN PARTICLES VOLUME PSA annexin A3 noninvasive prostate cancer self-normalization urine sensors