Kang, Ji Hee Kim, Kyoung-Ran Lee, Hyukjin Ahn, Dae-Ro Ko, Young Tag 2024-01-20T00:33:10Z 2024-01-20T00:33:10Z 2021-09-05 2017-09-01 0927-7765 https://pubs.kist.re.kr/handle/201004/122304 Deoxyribonucleic acid (DNA) is a versatile material with high applicability and inherent biocompatibility. L-DNA, the perfect mirror form of the naturally occurring D-DNA, has been used in DNA nanotechnology. It has thermodynamically identical properties to D-DNA, is capable of self-assembly and bio-orthogonal base-pairing, and is resistant to nuclease activity. We previously constructed an L-DNA tetrahedron (L-Td) and found that this nanostructure has remarkably higher capacity for cell penetration than its natural counterpart (D-Td). L-Td molecules of two different sizes one with 17-mer per side (L-Td(17)) and the other with 30-mer per side (L-Td(30)) were prepared by assembling four L-DNA strands. In this study, cellular uptake of L-Td with different sizes was observed over time using a laser scanning confocal microscope (LSCM) equipped with a live cell chamber system. In addition, we conducted a pharmacokinetic study to examine the potential of L-Td as a carrier for in vivo tumor-targeted delivery of a low dose of doxorubicin (DOX). L-Td entered into the cells through endocytosis, and a specific DNA sequence of the L-Td ensures targeted entry into cancer cells. Compared with free DOX, DOX-loaded L-Td (DOX@L-Td) showed decreased clearance and increased initial concentration (C-0), half-life, and area under the curve (AUC), indicating that DOX@L-Td circulated in the blood stream for longer than free DOX. L-Td(17), in particular, had beneficial effects owing to its ability to enhance tumor accumulation of DOX and reduce the cardiotoxicity caused by it through administration of a low dose of the drug. (C) 2017 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV ACUTE LYMPHOBLASTIC-LEUKEMIA DRUG-DELIVERY CANCER-CHEMOTHERAPY THERAPY NANOSTRUCTURES NANOTECHNOLOGY NANOPARTICLES LIPOSOMES TOXICITY SYSTEMS In vitro and in vivo behavior of DNA tetrahedrons as tumor-targeting nanocarriers for doxorubicin delivery Article 10.1016/j.colsurfb.2017.06.014 1 COLLOIDS AND SURFACES B-BIOINTERFACES, v.157, pp.424 - 431 COLLOIDS AND SURFACES B-BIOINTERFACES 157 424 431 scie scopus 000408597900049 2-s2.0-85020917070 Biophysics Chemistry, Physical Materials Science, Biomaterials Biophysics Chemistry Materials Science Article ACUTE LYMPHOBLASTIC-LEUKEMIA DRUG-DELIVERY CANCER-CHEMOTHERAPY THERAPY NANOSTRUCTURES NANOTECHNOLOGY NANOPARTICLES LIPOSOMES TOXICITY SYSTEMS DNA nanostructure DNA tetrahedron Tumor-targeted delivery Doxorubicin Live cell imaging