Jo, Suah Yoon, Jinkyung Lee, Sun-Mi Um, Youngsoon Han, Sung Ok Woo, Han Min 2024-01-20T00:33:30Z 2024-01-20T00:33:30Z 2021-09-05 2017-09 0168-1656 https://pubs.kist.re.kr/handle/201004/122321 Xylose-negative Corynebacterium glutamicum has been engineered to utilize xylose as the sole carbon source via either the xylose isomerase (XI) pathway or the Weimberg pathway. Heterologous expression of xylose isomerase and overexpression of a gene encoding for xylulose kinase enabled efficient xylose utilization. In this study, we show that two functionally-redundant transcriptional regulators (GntR1 and GntR2) present on xylose repress the pentose phosphate pathway genes. For efficient xylose utilization, pentose phosphate pathway genes and a phosphoketolase gene were overexpressed with the XI pathway in C. glutamicum. Overexpression of the genes encoding for transaldolase (Tal), 6-phosphogluconate dehydrogenase (Gnd), or phosphoketolase (XpkA) enhanced the growth and xylose consumption rates compared to the wild-type with the XI pathway alone. However, co-expression of these genes did not have a synergetic effect on xylose utilization. For the succinate production from xylose, overexpression of the tat gene with the XI pathway in a succinate-producing strain improved xylose utilization and increased the specific succinate production rate by 2.5-fold compared to wild type with the XI pathway alone. Thus, overexpression of the tal, gnd, or xpkA gene could be helpful for engineering C glutamicum toward production of value-added chemicals with efficient xylose utilization. (C) 2017 Elsevier B.V. All rights reserved. English Elsevier BV Modular pathway engineering of Corynebacterium glutamicum to improve xylose utilization and succinate production Article 10.1016/j.jbiotec.2017.01.015 1 Journal of Biotechnology, v.258, pp.69 - 78 Journal of Biotechnology 258 69 78 N scie scopus 000412611100010 2-s2.0-85011349337 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article ESCHERICHIA-COLI PHOSPHOKETOLASE PATHWAY SACCHAROMYCES-CEREVISIAE METABOLIC PATHWAY GENE-EXPRESSION CATABOLISM GLUCOSE GROWTH TRANSFORMATION GNTR1 Corynebacterium glutamicum Synthetic biology Metabolic engineering Xylose utilization Phosphoketolase