Min Kyoung Seon Yum, tae woo Kim Jiye Woo, Han Min Kim, Yunje Sang, Byoung-In Yoo, Young Je Kim, Yong Hwan Um, Youngsoon 2024-01-20T00:33:36Z 2024-01-20T00:33:36Z 2021-09-05 2017-09 1754-6834 https://pubs.kist.re.kr/handle/201004/122327 Background: In the biorefinery utilizing lignocellulosic biomasses, lignin decomposition to value-added phenolic derivatives is a key issue, and recently biocatalytic delignification is emerging owing to its superior selectivity, low energy consumption, and unparalleled sustainability. However, besides heme-containing peroxidases and laccases, information about lignolytic biocatalysts is still limited till date. Results: Herein, we report a promiscuous activity of tyrosinase which is closely associated with delignification requiring high redox potentials (>1.4 V vs. normal hydrogen electrode [NHE]). The promiscuous activity of tyrosinase not only oxidizes veratryl alcohol, a commonly used nonphenolic substrate for assaying ligninolytic activity, to veratraldehyde but also cleaves the 4-O-5 and C-alpha-C-beta bonds in 4-phenoxyphenol and guaiacyl glycerol-beta-guaiacyl ether (GGE) that are dimeric lignin model compounds. Cyclic voltammograms additionally verified that the promiscuous activity oxidizes lignin-related high redox potential substrates. Conclusion: These results might be applicable for extending the versatility of tyrosinase toward biocatalytic delignification as well as suggesting a new perspective for sustainable lignin utilization. Furthermore, the results provide insight for exploring the previously unknown promiscuous activities of biocatalysts much more diverse than ever thought before, thereby innovatively expanding the applicable area of biocatalysis. English BioMed Central Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and C-alpha-C-beta bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase Article 10.1186/s13068-017-0900-3 1 Biotechnology for Biofuels, v.10 Biotechnology for Biofuels 10 Y scie scopus 000410042600001 2-s2.0-85029177922 Biotechnology & Applied Microbiology Energy & Fuels Biotechnology & Applied Microbiology Energy & Fuels Article SITE-DIRECTED MUTAGENESIS RHODOCOCCUS-JOSTII RHA1 AGARICUS-BISPORUS L-DOPA PEROXIDASE OXIDATION ENZYMES DEGRADATION SPECIFICITY PATHWAYS Sustainable lignin utilization Tyrosinase Promiscuous activity 4-Phenoxyphenol Guaiacyl glycerol-beta-guaiacyl ether (GGE)