Kim, Seong Ho Kim, Kyoung-Ran Ahn, Dae-Ro Lee, Ji Eun Yang, Eun Gyeong Kim, So Yeon 2024-01-20T00:33:44Z 2024-01-20T00:33:44Z 2021-09-05 2017-09 1936-0851 https://pubs.kist.re.kr/handle/201004/122335 Reversible regulation of enzyme activity by chemical and physical stimuli is often achieved by incorporating stimuli-responsive domains in the enzyme of interest. However, this method is suitable for a limited number of enzymes with well-defined structural and conformational changes. In this study, we present a method to encapsulate enzymes in a DNA cage that could transform its conformation depending on the pH, allowing reversible control of the accessibility of the enzyme to the surrounding environment. This enabled us to regulate various properties of the enzyme, such as its resistance to protease-dependent degradation, binding affinity to the corresponding antibody, and most importantly, enzyme activity. Considering that the size and pH responsiveness of the DNA cage can be easily adjusted by the DNA length and sequence, our method provides a broad impact platform for controlling enzyme functions without modifying the enzyme of interest. English AMER CHEMICAL SOC MOLECULE PULL-DOWN 3-DIMENSIONAL STRUCTURE PROTEIN INTERACTIONS OPTOGENETIC CONTROL RIBONUCLEASE-A DRUG-DELIVERY RELEASE NANOSTRUCTURES TRANSCRIPTION PATHWAYS Reversible Regulation of Enzyme Activity by pH-Responsive Encapsulation in DNA Nanocages Article 10.1021/acsnano.7b04766 1 ACS NANO, v.11, no.9, pp.9352 - 9359 ACS NANO 11 9 9352 9359 scie scopus 000411918200086 2-s2.0-85029954954 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Article MOLECULE PULL-DOWN 3-DIMENSIONAL STRUCTURE PROTEIN INTERACTIONS OPTOGENETIC CONTROL RIBONUCLEASE-A DRUG-DELIVERY RELEASE NANOSTRUCTURES TRANSCRIPTION PATHWAYS DNA tetrahedron conformational change enzyme encapsulation reversible control enzyme activity