Shin, Jihye Song, Sang-Yun Ahn, Hee-Sung An, Byung Chull Choi, Yoo-Duk Yang, Eun Gyeong Na, Kook-Joo Lee, Seung-Taek Park, Jae-Il Kim, Seon-Young Lee, Cheolju Lee, Seung-won 2024-01-20T01:00:19Z 2024-01-20T01:00:19Z 2021-09-05 2017-08-24 1932-6203 https://pubs.kist.re.kr/handle/201004/122397 Non-small-cell lung cancer (NSCLC) constitutes approximately 80% of all diagnosed lung cancers, and diagnostic markers detectable in the plasma/serum of NSCLC patients are greatly needed. In this study, we established a pipeline for the discovery of markers using 9 transcriptome datasets from publicly available databases and profiling of six lung cancer cell secretomes. Thirty-one out of 312 proteins that overlapped between two-fold differentially expressed genes and identified cell secretome proteins were detected in the pooled plasma of lung cancer patients. To quantify the candidates in the serum of NSCLC patients, multiple- reaction-monitoring mass spectrometry (MRM-MS) was performed for five candidate biomarkers. Finally, two potential biomarkers (BCHE and GPx3; AUC = 0.713 and 0.673, respectively) and one two-marker panel generated by logistic regression (BCHE/GPx3; AUC = 0.773) were identified. A validation test was performed by ELISA to evaluate the reproducibility of GPx3 and BCHE expression in an independent set of samples (BCHE and GPx3; AUC = 0.630 and 0.759, respectively, BCHE/GPx3 panel; AUC = 0.788). Collectively, these results demonstrate the feasibility of using our pipeline for marker discovery and our MRM-MS platform for verifying potential biomarkers of human diseases. English PUBLIC LIBRARY SCIENCE GLUTATHIONE-PEROXIDASE GPX3 CHOLINESTERASE ACTIVITY BIOMARKER CANDIDATES MASS-SPECTROMETRY PROTEOMICS PREDICTION ADENOCARCINOMA QUANTIFICATION QUANTITATION VERIFICATION Integrative analysis for the discovery of lung cancer serological markers and validation by MRM-MS Article 10.1371/journal.pone.0183896 1 PLOS ONE, v.12, no.8 PLOS ONE 12 8 scie scopus 000408365000134 2-s2.0-85028370400 Multidisciplinary Sciences Science & Technology - Other Topics Article GLUTATHIONE-PEROXIDASE GPX3 CHOLINESTERASE ACTIVITY BIOMARKER CANDIDATES MASS-SPECTROMETRY PROTEOMICS PREDICTION ADENOCARCINOMA QUANTIFICATION QUANTITATION VERIFICATION