Jung, Seungwon Kim, Jungmin Kim, Junsun Yang, Sang Hwa Kim, Sang Kyung 2024-01-20T01:00:36Z 2024-01-20T01:00:36Z 2021-09-04 2017-08-15 0956-5663 https://pubs.kist.re.kr/handle/201004/122413 The early diagnosis of pathogenic bacteria is significant for bacterial identification and antibiotic resistance. Implementing rapid, sensitive, and specific detection, molecular diagnosis has been considered complementary to the conventional bacterial culture. Composite microparticles of a primer-immobilized network (cPIN) are developed for multiplex detection of pathogenic bacteria with real-time polymerase chain reaction (qPCR). A pair of specific primers are incorporated and stably conserved in a cPIN particle. One primer is crosslinked to the polymer network, and the other is bound to carbon nanotubes (CNTs) in the particle. At the initiation of qPCR, the latter primer is released from the CNTs and participates in the amplification. The amplification efficiency of this cPIN qPCR is estimated at more than 90% with suppressed non-specific signals from complex samples. In multiplexing, four infective pathogens are successfully discriminated using this cPIN qPCR. Multiplex qPCR conforms with the corresponding singleplex assays, proving independent amplification in each particle. Four bacterial targets from clinical samples are differentially analyzed in 30 min of a single qPCR trial with multiple cPIN particles. English ELSEVIER ADVANCED TECHNOLOGY POLYMERASE-CHAIN-REACTION MOLECULAR DIAGNOSTICS DNA PATHOGENS INFECTIONS ROUTINE HYBRIDS ASSAYS GENE Extensible multiplex real-time PCR for rapid bacterial identification with carbon nanotube composite microparticles Article 10.1016/j.bios.2017.02.049 1 BIOSENSORS & BIOELECTRONICS, v.94, pp.256 - 262 BIOSENSORS & BIOELECTRONICS 94 256 262 scie scopus 000401201300033 2-s2.0-85014805462 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article POLYMERASE-CHAIN-REACTION MOLECULAR DIAGNOSTICS DNA PATHOGENS INFECTIONS ROUTINE HYBRIDS ASSAYS GENE Hydrogels Carbon nanotubes Biomedical applications Multiplex real-time PCR Bacterial detection