Chae, Dong-Kyu Ban, Eunmi Yoo, Young Sook Kim, Eunice EunKyeong Baik, Ja-Hyun Song, Eun Joo 2024-01-20T01:02:08Z 2024-01-20T01:02:08Z 2021-09-04 2017-08 0899-1987 https://pubs.kist.re.kr/handle/201004/122499 The transforming growth factor- (TGF-) signaling pathway is associated with carcinogenesis and various biological processes. SMAD2 and SMAD4, which are putative tumor suppressors, have an important role in TGF- signaling. The aberrant expression of these genes is implicated in some cancers. However, the mechanisms of SMAD2 and SMAD4 dysregulation are poorly understood. In this study, we observed that miR-27a was upregulated in lung cancer cell lines and patients. In addition, SMAD2 and SMAD4 genes were identified as targets of miR-27a by several target prediction databases and experimental validation. Functional studies revealed that miR-27a overexpression decreased SMAD2 and SMAD4 mRNA and protein levels. Furthermore, miR-27a contributed to cell proliferation and invasion by inhibiting TGF--induced cell cycle arrest. These results suggest that miR-27a may function as an oncogene by regulating SMAD2 and SMAD4 in lung cancer. Thus, miR-27a may be a potential target for cancer therapy. English WILEY MICRORNA-27A FUNCTIONS TUMOR-SUPPRESSOR PROGRESSION CARCINOMA CELLS MIR-27a regulates the TGF- signaling pathway by targeting SMAD2 and SMAD4 in lung cancer Article 10.1002/mc.22655 1 MOLECULAR CARCINOGENESIS, v.56, no.8, pp.1992 - 1998 MOLECULAR CARCINOGENESIS 56 8 1992 1998 scie scopus 000404896500014 2-s2.0-85017505665 Biochemistry & Molecular Biology Oncology Biochemistry & Molecular Biology Oncology Article MICRORNA-27A FUNCTIONS TUMOR-SUPPRESSOR PROGRESSION CARCINOMA CELLS lung cancer miR-27a SMAD2 SMAD4 TGF-