Shin, Myoung-Sook Park, Jun Yeon Lee, Jaemin Yoo, Hye Hyun Hahm, Dae-Hyun Lee, Sang Cheon Lee, Sanghyun Hwang, Gwi Seo Jung, Kiwon Kang, Ki Sung 2024-01-20T01:02:35Z 2024-01-20T01:02:35Z 2021-09-04 2017-07-15 0960-894X https://pubs.kist.re.kr/handle/201004/122522 In this study, we investigated the anti-inflammatory effects and mechanisms of cirsimaritin isolated from an ethanol extract of the aerial parts of Cirsium japonicum var. maackii Maxim. using RAW264.7 cells. The extract and its flavonoid cirsimaritin inhibited nitric oxide (NO) production and inducible nitric oxide synthase expression in RAW264.7 cells. Cirsimaritin inhibited interleukin-6, tumor necrosis factor-alpha, and NO production in a concentration-dependent manner in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. From a western blot study, pretreatment with cirsimaritin inhibited phosphorylation/degradation of I kappa B alpha and phosphorylation of Akt in LPS-stimulated RAW264.7 cells. Moreover, cirsimaritin suppressed activation of LPS-induced transcription factors, such as c-fos and signal transducer and activator of transcription 3 (STAT3), in RAW264.7 cells. Collectively, these results show that cirsimaritin possesses anti-inflammatory activity, which is regulated by inhibition of c-fos and STAT3 phosphorylation in RAW264.7 cells. (C) 2017 Elsevier Ltd. All rights reserved. English Pergamon Press Ltd. NF-KAPPA-B CYTOKINE PRODUCTION OXIDATIVE STRESS IN-VITRO INFLAMMATION DERIVATIVES INHIBITION MODULATION ACTIVATION DISEASE Anti-inflammatory effects and corresponding mechanisms of cirsimaritin extracted from Cirsium japonicum var. maackii Maxim Article 10.1016/j.bmcl.2017.05.051 1 Bioorganic & Medicinal Chemistry Letters, v.27, no.14, pp.3076 - 3080 Bioorganic & Medicinal Chemistry Letters 27 14 3076 3080 scie scopus 000404422000008 2-s2.0-85019956525 Chemistry, Medicinal Chemistry, Organic Pharmacology & Pharmacy Chemistry Article NF-KAPPA-B CYTOKINE PRODUCTION OXIDATIVE STRESS IN-VITRO INFLAMMATION DERIVATIVES INHIBITION MODULATION ACTIVATION DISEASE Cirsium japonicum Cirsimaritin Inflammation