Park, JinSeok Holmes, William R. Lee, Sung Hoon Kim, Hong-Nam Kim, Deok-Ho Kwak, Moon Kyu Wang, Chiaochun Joanne Edelstein-Keshet, Leah Levchenko, Andre 2024-01-20T01:02:42Z 2024-01-20T01:02:42Z 2021-09-05 2017-07-11 0027-8424 https://pubs.kist.re.kr/handle/201004/122528 Cell polarization and directional cell migration can display random, persistent, and oscillatory dynamic patterns. However, it is not clear whether these polarity patterns can be explained by the same underlying regulatory mechanism. Here, we show that random, persistent, and oscillatory migration accompanied by polarization can simultaneously occur in populations of melanoma cells derived from tumors with different degrees of aggressiveness. We demonstrate that all of these patterns and the probabilities of their occurrence are quantitatively accounted for by a simple mechanism involving a spatially distributed, mechanochemical feedback coupling the dynamically changing extracellular matrix (ECM)-cell contacts to the activation of signaling downstream of the Rho-family small GTPases. This mechanism is supported by a predictive mathematical model and extensive experimental validation, and can explain previously reported results for diverse cell types. In melanoma, this mechanism also accounts for the effects of genetic and environmental perturbations, including mutations linked to invasive cell spread. The resulting mechanistic understanding of cell polarity quantitatively captures the relationship between population variability and phenotypic plasticity, with the potential to account for a wide variety of cell migration states in diverse pathological and physiological conditions. English NATL ACAD SCIENCES EXTRACELLULAR-MATRIX INTEGRIN ALPHA-V-BETA-3 SIGNALING PATHWAYS MEMBRANE TENSION CANCER-CELLS RHO-GTPASES MIGRATION ADHESION INVASION MELANOMA Mechanochemical feedback underlies coexistence of qualitatively distinct cell polarity patterns within diverse cell populations Article 10.1073/pnas.1700054114 1 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.114, no.28, pp.E5750 - E5759 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114 28 E5750 E5759 scie scopus 000405177100032 2-s2.0-85023162493 Multidisciplinary Sciences Science & Technology - Other Topics Article EXTRACELLULAR-MATRIX INTEGRIN ALPHA-V-BETA-3 SIGNALING PATHWAYS MEMBRANE TENSION CANCER-CELLS RHO-GTPASES MIGRATION ADHESION INVASION MELANOMA cell polarization cell migration mechanochemical feedback Rho-family small GTPases extracellular matrix