Rahman, Md. Ataur Kim, Hanna Lee, Kang Ho Yun, Hyung-Mun Hong, Jung-Hwa Kim, Youngjae Choo, Hyunah Park, Mikyoung Rhim, Hyewhon 2024-01-20T01:03:32Z 2024-01-20T01:03:32Z 2021-09-05 2017-07 1016-8478 https://pubs.kist.re.kr/handle/201004/122572 The 5-HT6R has been considered as an attractive therapeutic target in the brain due to its exclusive expression in the brain. However, the mechanistic linkage between 5-HT(6)Rs and brain functions remains poorly understood. Here, we examined the effects of 5-HT6R-mediated cell morphological changes using immunocytochemistry, Western blot, and live-cell imaging assays. Our results showed that the activation of 5-HT(6)Rs caused morphological changes and increased cell surface area in HEK293 cells expressing 5-HT(6)Rs. Treatment with 5-HT specifically increased RhoA-GTP activity without affecting other Rho family proteins, such as Rac1 and Cdc42. Furthermore, live-cell imaging in hippocampal neurons revealed that activation of 5-HT(6)Rs using a selective agonist, ST1936, increased the density and size of dendritic protrusions along with the activation of RhoA-GTP activity and that both effects were blocked by pretreatment with a selective 5-HT6R antagonist, SB258585. Taken together, our results show that 5-HT6R plays an important role in the regulation of cell morphology via a RhoA-dependent pathway in mammalian cell lines and primary neurons. English KOREAN SOC MOLECULAR & CELLULAR BIOLOGY PHYSICAL INTERACTION DENDRITIC SPINES 5-HT6 RECEPTORS SMALL GTPASES SEROTONIN PLASTICITY ACTIVATION MECHANISM NEURONS 5-Hydroxytryptamine 6 Receptor (5-HT6R)-Mediated Morphological Changes via RhoA-Dependent Pathways Article 10.14348/molcells.2017.0080 1 MOLECULES AND CELLS, v.40, no.7, pp.495 - 502 MOLECULES AND CELLS 40 7 495 502 scie scopus kci ART002247587 000412952400007 2-s2.0-85029482643 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article PHYSICAL INTERACTION DENDRITIC SPINES 5-HT6 RECEPTORS SMALL GTPASES SEROTONIN PLASTICITY ACTIVATION MECHANISM NEURONS 5-HT6R dendritic protrusions live-cell imaging morphology serotonin RhoA-GTP