Heo, Roun You, Dong Gil Um, Wooram Choi, Ki Young Jeon, Sangmin Park, Jong-Sung Choi, Yuri Kwon, Seunglee Kim, Kwangmeyung Kwon, Ick Chan Jo, Dong-Gyu Kang, Young Mo Park, Jae Hyung 2024-01-20T01:03:34Z 2024-01-20T01:03:34Z 2021-09-05 2017-07 0142-9612 https://pubs.kist.re.kr/handle/201004/122574 With the aim of developing nanoparticles for targeted delivery of methotrexate (MTX) to inflamed joints in rheumatoid arthritis (RA), an amphiphilic polysaccharide was synthesized by conjugating 5 beta-cholanic acid to a dextran sulfate (DS) backbone. Due to its amphiphilic nature, the DS derivative self-assembled into spherical nanoparticles (220 nm in diameter) in aqueous conditions. The MTX was effectively loaded into the DS nanoparticles (loading efficiency: 73.0%) by a simple dialysis method. Interestingly, the DS nanoparticles were selectively taken up by activated macrophages, which are responsible for inflammation and joint destruction, via scavenger receptor class A-mediated endocytosis. When systemically administrated into mice with experimental collagen-induced arthritis (CIA), the DS nanoparticles effectively accumulated in inflamed joints (12-fold more than wild type mice (WT)), implying their high targetability to RA tissues. Moreover, the MTX-loaded DS nanoparticles exhibited significantly improved therapeutic efficacy against CIA in mice compared to free MTX alone. Overall, the data presented here indicate that DS nanoparticles are potentially useful nanomedicines for RA imaging and therapy. (C) 2017 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD HYALURONIC-ACID NANOPARTICLES MACROPHAGE SCAVENGER RECEPTOR COLLAGEN-INDUCED ARTHRITIS IN-VIVO VITRO ATHEROSCLEROSIS DOXORUBICIN CYTOKINES DELIVERY THERAPY Dextran sulfate nanoparticles as a theranostic nanomedicine for rheumatoid arthritis Article 10.1016/j.biomaterials.2017.03.044 1 BIOMATERIALS, v.131, pp.15 - 26 BIOMATERIALS 131 15 26 scie scopus 000401393600002 2-s2.0-85016478906 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article HYALURONIC-ACID NANOPARTICLES MACROPHAGE SCAVENGER RECEPTOR COLLAGEN-INDUCED ARTHRITIS IN-VIVO VITRO ATHEROSCLEROSIS DOXORUBICIN CYTOKINES DELIVERY THERAPY Dextran sulfate Nanoparticles Drug delivery Macrophage Rheumatoid arthritis