Murale, Dhiraj P. Hong, Seong Cheol Haque, Md. Mamunul Lee, Jun-Seok 2024-01-20T01:04:19Z 2024-01-20T01:04:19Z 2022-01-10 2017-06-24 1477-5956 https://pubs.kist.re.kr/handle/201004/122614 Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs. English BIOMED CENTRAL LTD CARBOHYDRATE-BINDING PROTEIN SITE-SPECIFIC INCORPORATION UNNATURAL AMINO-ACIDS CELL-CULTURE SILAC CROSS-LINKING PHOTOAFFINITY PROBES LIVING CELLS IN-VIVO ESCHERICHIA-COLI GENETIC-CODE Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs) Article 10.1186/s12953-017-0123-3 1 PROTEOME SCIENCE, v.15 PROTEOME SCIENCE 15 scie scopus 000405227600002 2-s2.0-85021275870 Biochemical Research Methods Biochemistry & Molecular Biology Review CARBOHYDRATE-BINDING PROTEIN SITE-SPECIFIC INCORPORATION UNNATURAL AMINO-ACIDS CELL-CULTURE SILAC CROSS-LINKING PHOTOAFFINITY PROBES LIVING CELLS IN-VIVO ESCHERICHIA-COLI GENETIC-CODE Photo-affinity probe Protein-protein interaction Quantitative proteomics Benzophenone Aryl azide Diazirine