Kim, Minkyung Hong, Jinki Kim, Jinsik Shin, Hyun-joon 2024-01-20T01:30:30Z 2024-01-20T01:30:30Z 2021-09-05 2017-06-01 2156-7085 https://pubs.kist.re.kr/handle/201004/122650 We report a fiber optics-based intravital fluorescence imaging platform that includes epi-fluorescence microscopy and laser patterned-light stimulation system. The platform can perform real-time fluorescence imaging with a lateral resolution of similar to 4.9 mu m while directly inserted into the intact mouse brain, optically stimulate vasoconstriction during real-time imaging, and avoid vessel damage in the penetration path of imaging probe. Using 473-nm patterned-light stimulation, we successfully modulated the vasoconstriction of a single targeted 37-mu m-diameter blood vessel located more than 4.7 mm below the brain surface of a live SM22-ChR2 mouse. This platform may permit the hemodynamic studies associated with deeper brain neurovascular disorders. (C) 2017 Optical Society of America English OPTICAL SOC AMER OPTOGENETIC MODULATION ELECTRICAL-STIMULATION HEMODYNAMIC-RESPONSE BLOOD-FLOW MICROSCOPY CORTEX Fiber bundle-based integrated platform for widefield fluorescence imaging and patterned optical stimulation for modulation of vasoconstriction in the deep brain of a living animal Article 10.1364/BOE.8.002781 1 BIOMEDICAL OPTICS EXPRESS, v.8, no.6, pp.2781 - 2795 BIOMEDICAL OPTICS EXPRESS 8 6 2781 2795 scie scopus 000404737200001 2-s2.0-85020221435 Biochemical Research Methods Optics Radiology, Nuclear Medicine & Medical Imaging Biochemistry & Molecular Biology Optics Radiology, Nuclear Medicine & Medical Imaging Article OPTOGENETIC MODULATION ELECTRICAL-STIMULATION HEMODYNAMIC-RESPONSE BLOOD-FLOW MICROSCOPY CORTEX Blood or tissue constituent monitoring Fiber optics imaging Fluorescence microscopy Integrated optics