Shim, Kyuyoung Kim, So Hyun Lee, Dongwook Kim, Bumsang Kim, Tae Hee Jung, Youngmee Choi, Nakwon Sung, Jong Hwan 2024-01-20T01:31:09Z 2024-01-20T01:31:09Z 2021-09-05 2017-06 1226-086X https://pubs.kist.re.kr/handle/201004/122686 In hydrogel scaffolds for 3D cell culture, porous structure plays a central role in maintaining cell survival by delivery of biochemical factors through the scaffolds. Thus, adequate pore structures should be a significant design factor for preparing cell culture scaffolds. Here, we present a non-cytotoxic approach to control porosity of a gelatin scaffold based on porogen leaching. Specifically, we fabricated photocrosslinkable hydrogel (gelatin methacrylate) containing porogens in a microfluidic channel, followed by dissolving out the porogens. Our approach allowed generation of micrometer-scale pores, increasing cell viability. This study could serve as a guide for tuning porosity of cell-seeded 3D scaffolds. (C) 2017 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved. English 한국공업화학회 Fabrication of micrometer-scale porous gelatin scaffolds for 3D cell culture Article 10.1016/j.jiec.2017.02.012 1 Journal of Industrial and Engineering Chemistry, v.50, pp.183 - 189 Journal of Industrial and Engineering Chemistry 50 183 189 N scie scopus kci ART002231307 000399259500022 2-s2.0-85014059234 Chemistry, Multidisciplinary Engineering, Chemical Chemistry Engineering Article PART 1 HYDROGELS MICROSPHERES NETWORKS PLATFORM MODEL Gelatin-methacrylate (Gel-MA) Micrometer-scale porous hydrogel scaffold Photocrosslinking Porogen 3D cell culture