Lee, Hyojin Kim, Chloe Lee, Dongjin Park, Jea Ho Searson, Peter C. Lee, Kwan Hyi 2024-01-20T01:31:18Z 2024-01-20T01:31:18Z 2021-09-04 2017-06 1176-9114 https://pubs.kist.re.kr/handle/201004/122695 Recent studies have found that prostate cancer expresses abnormal genetic markers including multiple types of TMPRSS2-ERG fusion genes. The expression level of different TMPRSS2-ERG fusion genes is correlated to pathologic variables of aggressive prostate cancer and disease progression. State-of-the-art methods for detection of TMPRSS2-ERG fusion genes include reverse transcription polymerase chain reaction (RT-PCR) with a detection limit of 1 fmol at urinary condition. RT-PCR is time consuming, costly, and inapplicable for multiplexing. Ability to identify multiple fusion genes in a single sample has become important for diagnostic and clinical purposes. There is a need for a sensitive diagnostic test to detect multiple TMPRSS2-ERG fusion genes for an early diagnosis and prognosis of prostate cancer. Here, we propose to develop an assay for prostate cancer diagnosis using oligonucleotide-functionalized quantum dot and magnetic microparticle for optical detection of rearranged TMPRSS2-ERG fusion genes at a low concentration in urine. We found that our assay was able to identify three different types of fusion gene with a wide detection range and detection limit of 1 fmol (almost the same level of the RT-PCR result reported). Here, we show detection of multiple TMPRSS2-ERG fusion genes using color-coded oligonucleotides in cell lysate and urine. English DOVE MEDICAL PRESS LTD MOLECULAR CHARACTERIZATION CHEMISTRY Optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis Article 10.2147/IJN.S138081 1 INTERNATIONAL JOURNAL OF NANOMEDICINE, v.12, pp.4397 - 4407 INTERNATIONAL JOURNAL OF NANOMEDICINE 12 4397 4407 scie scopus 000403229800003 2-s2.0-85020934654 Nanoscience & Nanotechnology Pharmacology & Pharmacy Science & Technology - Other Topics Pharmacology & Pharmacy Article MOLECULAR CHARACTERIZATION CHEMISTRY fusion genes prostate cancer quantum dots multiplexed assay optical detection