Lee, So Young Kim, Joo Yeon Jung, Yu-Jin Kang, Kyungsu 2024-01-20T01:31:34Z 2024-01-20T01:31:34Z 2021-09-01 2017-06 1520-4081 https://pubs.kist.re.kr/handle/201004/122710 Etoposide, a topoisomerase II inhibitor, has been widely used as a clinical anticancer drug to treat diverse cancer patients. Since not only rapidly dividing cancer cells but also the cells of normal human tissues and every living organism in environmental ecosystems have topoisomerases, it is crucial to study the toxicity of etoposide in other organisms in addition to cancer cells. In this study, we evaluated the toxicity of etoposide in both a soil nematode, Caenorhabditis elegans, and 3T3-L1 normal murine cells. Etoposide significantly retarded the growth, egg laying, and hatching in C. elegans. Etoposide also affected the reproductive gonad tissue, decreased the number of germ cells and induced abnormally enlarged nuclei in C. elegans. In addition, etoposide inhibited 3T3-L1 cell proliferation, with IC50 values of 37.8 +/- 7.3 and 9.8 +/- 1.8 M after 24 and 48 hours of treatment, respectively, via the induction of cell cycle arrest at the G2/M phase and apoptotic cell death. Etoposide also induced nuclear enlargement in 3T3-L1 normal murine cells. The reproductive toxicity and abnormal nuclear morphological changes seemed to correlate with the adverse effects of etoposide. We suggest that these experimental platforms, i.e., the toxicological evaluation of both nematodes and 3T3-L1 cells, may be useful to study the mechanisms underlying the side effects of chemicals, including topoisomerase inhibitors. English WILEY EXTENDS LIFE-SPAN ANTINEOPLASTIC DRUGS II INHIBITORS TOXICITY PROLIFERATION DAURINOL PHASE WATER GENE Toxicological evaluation of the topoisomerase inhibitor, etoposide, in the model animal Caenorhabditis elegans and 3T3-L1 normal murine cells Article 10.1002/tox.22406 1 ENVIRONMENTAL TOXICOLOGY, v.32, no.6, pp.1836 - 1843 ENVIRONMENTAL TOXICOLOGY 32 6 1836 1843 scie scopus 000400996000016 2-s2.0-85013290424 Environmental Sciences Toxicology Water Resources Environmental Sciences & Ecology Toxicology Water Resources Article EXTENDS LIFE-SPAN ANTINEOPLASTIC DRUGS II INHIBITORS TOXICITY PROLIFERATION DAURINOL PHASE WATER GENE abnormal nuclei C elegans cellular toxicity etoposide germ cell toxicity reproductive toxicity topoisomerase inhibitor 3T3-L1 cells