Lim, Jung-hee Song, Mi-Kyung Cho, Yoon Kim, Woong Han, Sung Ok Ryu, Jae-Chun 2024-01-20T01:31:35Z 2024-01-20T01:31:35Z 2021-09-01 2017-06 0887-2333 https://pubs.kist.re.kr/handle/201004/122711 Recent studies have illustrated the growing importance of exosomes (small extracellular vesicles) and their constituent microRNAs (miRNAs) in the fields of toxicology and pathology. The mechanism of toxicity of toluene, a highly-prevalent and volatile organic compound, is largely unknown. To examine the role of miRNAs in toluene induced toxicity, we investigated miRNAs and toluene-induced gene expression in HL-60 human promyelocytic leukemia cells and exosomes using microarrays. A total of 54 miRNAs were differentially expressed in HL-60 cell lines exposed to toluene and exosomes from the cells. Four out of the 54 miRNAs (hsa-miR-1290, hsa-miR-718, hsa-miR-3663-3p, and hsa-miR-320c) were subsequently validated by qRT-PCR. Integrated analysis of miRNA and mRNA expression profiles identified 8 miRNA-mRNA correlations. By performing Comparative Toxicogenomics Database analysis, we found that the eight putative target genes of the differentially expressed miRNAs under toluene exposure (EXOSC6, RHOH, GFER, HERC2, GOLGA4, SLC7A11, GCLM, and BACH1) are related to diverse disease categories such as nervous system disease, cancer, cardiovascular disease, and respiratory tract disease. In conclusion, our data demonstrated that miRNA-mRNA networks provide a better understanding of toxicological mechanism caused by environmental pollutants in vitro using HL-60 cells and exosomes. (C) 2017 Elsevier Ltd. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD CENTRAL-NERVOUS-SYSTEM INDOOR AIR-POLLUTION OXIDATIVE STRESS CANCER MIRNA HEALTH BACH1 ACTIVATION BIOMARKERS MECHANISM Comparative analysis of microRNA and mRNA expression profiles in cells and exosomes under toluene exposure Article 10.1016/j.tiv.2017.02.020 1 TOXICOLOGY IN VITRO, v.41, pp.92 - 101 TOXICOLOGY IN VITRO 41 92 101 scie scopus 000404701800011 2-s2.0-85014415435 Toxicology Toxicology Article CENTRAL-NERVOUS-SYSTEM INDOOR AIR-POLLUTION OXIDATIVE STRESS CANCER MIRNA HEALTH BACH1 ACTIVATION BIOMARKERS MECHANISM Toluene Exosome HL-60 MicroRNA Target mRNA Microarray