Jung, J.H. Seo, Y.W. 2024-01-20T01:31:41Z 2024-01-20T01:31:41Z 2021-08-31 2017-06 1975-9479 https://pubs.kist.re.kr/handle/201004/122716 Genome editing technologies using customizable sequence specific nucleases (SSNs) enables precise genome modification in plants, which has revolutionized functional genomics research as well as crop improvement. Applicability of SSN-mediated genome editing ranges from targeted gene knockout and single base modification to multiple gene stacking into desired genomic sites. However, there are still considerable challenges in implementing genome editing technologies for practical crop improvement. Here, we briefly discuss the technological challenges, especially those associated with the delivery of SSNs and with homologous recombination-mediated genome editing, and address some promising solutions to overcome the issues. ？ 2017, Korean Society of Crop Science and Springer Science+Business Media B.V. English Korean Society of Crop Science Challenges in wide implementation of genome editing for crop improvement Article 10.1007/s12892-017-0019-0 1 Journal of Crop Science and Biotechnology, v.20, no.2, pp.129 - 135 Journal of Crop Science and Biotechnology 20 2 129 135 scopus kci ART002230648 2-s2.0-85021417078 Article crop improvement Genome editing homologous recombination (HR) sequence specific nucleases (SSNs)