Wang, Danyang Du, Shubo Cazenave-Gassiot, Amaury Ge, Jingyan Lee, Jun-Seok Wenk, Markus R. Yao, Shao Q. 2024-01-20T01:32:06Z 2024-01-20T01:32:06Z 2021-08-31 2017-05-15 1433-7851 https://pubs.kist.re.kr/handle/201004/122737 The protein-lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein-lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne-containing choline head group and a diazirine-modified fatty acid simultaneously into choline-containing phospholipids synthesized from live mammalian cells, protein-phospholipid interactions have been successfully imaged in live cells. Subsequent in situ profiling of the modified Cho phospholipid-crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid-interacting proteins, some of which were further validated. English John Wiley & Sons Ltd. PHOTO-CROSS-LINKERS MAMMALIAN-CELLS DISULFIDE-ISOMERASE IN-VIVO LINKING BIOLOGY Global Mapping of Protein-Lipid Interactions by Using Modified Choline-Containing Phospholipids Metabolically Synthesized in Live Cells Article 10.1002/anie.201702509 1 Angewandte Chemie International Edition, v.56, no.21, pp.5829 - 5833 Angewandte Chemie International Edition 56 21 5829 5833 scie scopus 000400755800031 Chemistry, Multidisciplinary Chemistry Article PHOTO-CROSS-LINKERS MAMMALIAN-CELLS DISULFIDE-ISOMERASE IN-VIVO LINKING BIOLOGY click chemistry phosphatidylcholine photo-crosslinking protein-lipid interactions quantitative proteomics