Ahn, Huijeong Jeon, Eunsaem Kim, Jin-Chul Kang, Seung Goo Yoon, Sung-il Ko, Hyun-Jeong Kim, Pyeung-Hyeun Lee, Geun-Shik 2024-01-20T01:32:28Z 2024-01-20T01:32:28Z 2021-09-01 2017-05-02 2045-2322 https://pubs.kist.re.kr/handle/201004/122757 Lentinan extracted from shiitake (Lentinula edodes) is a beta-glucan that has been reported as an intravenous anti-tumor polysaccharide via enhancement of the host immune system. In this study, we determined the effect of lentinan on inflammasome activation, a multi-protein platform, in myeloid cells. Mouse bone marrow-derived macrophages were treated with lentinan with/without inflammasome triggers, and maturation of interleukin (IL)-1 beta, IL-18, or caspase-1 was measured as a readout of inflammasome activation. As a result, lentinan selectively inhibited absent in melanoma 2 (AIM2) inflammasome activation. In addition, lentinan up-regulated pro-inflammatory cytokines and induced expression of inflammasome-related genes through toll-like receptor 4 signaling. Furthermore, we assessed the effect of lentinan on mice treated with Listeria monocytogenes or lipopolysaccharide as an AIM2 or non-canonical inflammasome-mediated model. Lentinan attenuated IL-1 beta secretion resulting from Listeria-mediated AIM2 inflammasome activation and reduced endotoxin lethality via inhibition of non-canonical inflammasome activation. Thus, lentinan is suggested as an anti-AIM2 and anti-non-canonical inflammasome candidate despite its up-regulation of cytokine expression. English NATURE PUBLISHING GROUP NLRP3 INFLAMMASOME CELL-PROLIFERATION IN-VITRO KAPPA-B MACROPHAGES MECHANISM INFECTION PROTEINS NLRC4 Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production Article 10.1038/s41598-017-01462-4 1 SCIENTIFIC REPORTS, v.7 SCIENTIFIC REPORTS 7 scie scopus 000400449000026 2-s2.0-85018250028 Multidisciplinary Sciences Science & Technology - Other Topics Article NLRP3 INFLAMMASOME CELL-PROLIFERATION IN-VITRO KAPPA-B MACROPHAGES MECHANISM INFECTION PROTEINS NLRC4 Lentinan AIM2 Inflammation IL-1 caspase-1