Kim, Seong-Eun Jo, Sung Duk Kwon, Koo Chul Won, You-Yeon Lee, Jeewon 2024-01-20T01:32:41Z 2024-01-20T01:32:41Z 2022-01-25 2017-05 2198-3844 https://pubs.kist.re.kr/handle/201004/122767 Hepatitis B virus capsid (HBVC), a self-assembled protein nanoparticle comprised of 180 or 240 subunit proteins, is used as a cage for genetic encapsulation of fluorescent proteins (FPs). The self-quenching of FPs is controlled by varying the spacing between FPs within the capsid structure. Double-layered FP nanoparticle possessing cancer cell-targeting capabilities is also produced by additionally attaching FPs and cancer cell receptor-binding peptides (affibodies) to the outer surface of the capsid. The generically modified HBVC with double layers of mCardinal FPs and affibodies (mC-DL-HBVC) exhibit a high fluorescence intensity and a strong photostability, and is efficiently internalized by cancer cells and significantly stable against intracellular degradation. The mC-DL-HBVC effectively detects tumor in live mice with enhanced tumor targeting and imaging efficiency with far less accumulation in the liver, compared to a conventional fluorescent dye, Cy5.5. This suggests the great potential of mC-DL-HBVC as a promising contrast agent for in vivo tumor fluorescence imaging. English WILEY Genetic Assembly of Double-Layered Fluorescent Protein Nanoparticles for Cancer Targeting and Imaging Article 10.1002/advs.201600471 1 ADVANCED SCIENCE, v.4, no.5 ADVANCED SCIENCE 4 5 N scie scopus 000402740300009 2-s2.0-85013272393 Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Article VIRAL NANOPARTICLES GUIDED SURGERY PARTICLES cancer targeting and imaging double-layered fluorescent proteins genetic encapsulation super-fluorescent protein nanoparticles viral capsid