Ezzat, Shahira M. Salama, Maha M. Mahrous, Engy A. Maes, Louis Pan, Cheol-Ho Abdel-Sattar, Essam 2024-01-20T01:32:50Z 2024-01-20T01:32:50Z 2021-09-05 2017-05 1478-6419 https://pubs.kist.re.kr/handle/201004/122775 The bioactive petroleum ether fraction of Verbesina encelioides, previously studied by the authors, was chosen for the isolation of antiprotozoal metabolites. Pseudotaraxasterol-3 beta-acetate (1), benzyl 2,6-dimethoxy benzoate (2), 16 beta-hydroxy-pseudotaraxasterol-3 beta-palmitate (3) and pseudotaraxasterol (4), in addition to beta-sitosterol glucoside (5) and beta-sitosterol galactoside (6) were isolated and identified based on one-dimensional and two-dimensional spectral analysis. This is the first report describing (3) and (6) in genus Verbesina. The isolated compounds were tested in vitro against Plasmodium falciparum, Trypanosoma brucei, Trypanosoma cruzi and Leishmania infantum. Cytotoxicity was evaluated on MRC-5 cells. Compound 1 showed moderate to weak activity against L. infantum T. brucei and P. falciparum and was inactive against T. cruzi. Compound 3 showed moderate activity against L. infantum, compound 4 revealed weak activity against T. cruzi, while 5 and 6 were inactive against all tested protozoa. All compounds were non-cytotoxic. The isolated constituents showed less antiprotozoal activity than the crude fraction. [GRAPHICS] . English TAYLOR & FRANCIS LTD CHAGAS-DISEASE MALARIA Antiprotozoal activity of major constituents from the bioactive fraction of Verbesina encelioides Article 10.1080/14786419.2016.1180604 1 NATURAL PRODUCT RESEARCH, v.31, no.6, pp.676 - 680 NATURAL PRODUCT RESEARCH 31 6 676 680 scie scopus 000392629600010 2-s2.0-84965029156 Chemistry, Applied Chemistry, Medicinal Chemistry Pharmacology & Pharmacy Article CHAGAS-DISEASE MALARIA Antimalarial antitrypanosomal antileishmanial cytotoxicity