Kim, Hyunju Woo, Dustin J. Kim, So Yeon Yang, Eun Gyeong 2024-01-20T01:33:50Z 2024-01-20T01:33:50Z 2021-09-01 2017-04-29 0006-291X https://pubs.kist.re.kr/handle/201004/122830 The p21-activated kinases (Paks) interact with Rac/Cdc42 GTPases to regulate the actin cytoskeleton as well as various signaling pathways. Although activation of Paks in many human cancers is known to mediate cancer progression, the role of Pak proteins in hypoxia is poorly understood. In this study, we found that both Pakl and Pak4 are highly expressed in HeLa cervical cancer cells, but only Pak4 knockdown attenuates expression of hypoxia-inducible factor-1 alpha (HIF-1 alpha) in hypoxia. We further discovered that Pak4 regulates HIF-l alpha translation via the Akt-mTOR-4E-BP1 pathway under hypoxic conditions. These results support a novel connection between HIF-la and Pak4 in hypoxic cancer cells, and provide insights into mechanisms whereby tumors respond to and thrive under oxygen-deficient conditions. (C) 2017 Elsevier Inc. All rights reserved. English ACADEMIC PRESS INC ELSEVIER SCIENCE CISPLATIN-RESISTANCE PROTEIN-KINASES PAK4 CONFERS HYPOXIA ACTIVATION EXPRESSION ERK SURVIVAL TARGET 4E-BP1 p21-activated kinase 4 regulates HIF-1 alpha translation in cancer cells Article 10.1016/j.bbrc.2017.03.024 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.486, no.2, pp.270 - 276 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 486 2 270 276 scie scopus 000399261200010 2-s2.0-85015005140 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article CISPLATIN-RESISTANCE PROTEIN-KINASES PAK4 CONFERS HYPOXIA ACTIVATION EXPRESSION ERK SURVIVAL TARGET 4E-BP1 Pak4 HIF-l alpha Hypoxia Akt mTOR 4E-BP1