Shin, Hyun Ju Park, Na Hyun Lee, Wonwoong Choi, Man Ho Chung, Bong Chul Hong, Jongki 2024-01-20T01:34:06Z 2024-01-20T01:34:06Z 2021-09-01 2017-04-15 1570-0232 https://pubs.kist.re.kr/handle/201004/122843 The tyrosine, tryptophan, and glutamate metabolic pathways play key roles on pathological state of neuronal functions and the change of their levels in biological systems reflects the progress degree of neuronal diseases. Comprehensive profiling of these metabolites is important to find new biomarkers for diagnosis or prognosis of various neuronal diseases. However, the overall profiling analysis of various neurochemicals in biological sample is confronted with several limitations due to their low concentration and physicochemical properties and the coexistence of matrices. We developed an efficient and feasible method using gas chromatography-tandem mass spectrometry (GC-MS/MS). Wide-bore mixed cation exchange (MCX) SPE process enables a rapid and effective cleanup of 20 neurochemicals even including acidic and basic neurochemicals in a single SPE cartridge by using different composition of eluents. Selective derivatization of various types of metabolites was applied to achieve highly chromatographic separation and sensitive mass detection. Appropriate selection of precursor and product transition ions used in multiple reaction-monitoring (MRM) mode based on the MS/MS fragmentations of the derivatized neurochemicals could be significantly minimized the matrix effects and enhanced the reliability of quantification results. The developed method was validated in terms of linearity, limits of detection, precision, accuracy, and matrix effects. The intra-and inter-assay analytical variations were less than 10%. The overall linearity for all of the targets was excellent (R-2 >= 0.996). The detection limits ranged between 0.38 and 8.13 ng/mL for the acidic neurochemicals and between 0.02 and 11.1 ng/mL for the basic neurochemicals. The developed protocol will be expected to be a promising tool for the understanding of the pathological state and diagnosis of various neuronal diseases. (C) 2017 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV LIQUID-CHROMATOGRAPHY ALZHEIMERS-DISEASE PHASE MICROEXTRACTION ACIDIC METABOLITES BRAIN CATECHOLAMINES PERFORMANCE DOPAMINE PLASMA NEUROTRANSMITTERS Metabolic profiling of tyrosine, tryptophan, and glutamate in human urine using gas chromatography-tandem mass spectrometry combined with single SPE cleanup Article 10.1016/j.jchromb.2017.03.015 1 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.1051, pp.97 - 107 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1051 97 107 scie scopus 000399510800013 2-s2.0-85015749131 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article LIQUID-CHROMATOGRAPHY ALZHEIMERS-DISEASE PHASE MICROEXTRACTION ACIDIC METABOLITES BRAIN CATECHOLAMINES PERFORMANCE DOPAMINE PLASMA NEUROTRANSMITTERS Neurotransmitters Acidic hydrolysis MCX-SPE Chemical derivatization GC-MS/MS Neuronal diseases