Kim, Yu Ra Pyo, Hee Soo Chung, Bong Chul Moon, Myeong Hee Lee, Jeongae 2024-01-20T02:00:18Z 2024-01-20T02:00:18Z 2021-09-01 2017-04 0253-2964 https://pubs.kist.re.kr/handle/201004/122883 This study aimed to measure phytoestrogen contents in health functional foods (HFFs) to alleviate menopausal symptoms that are commercially available in South Korea. A sensitive and selective method using gas chromatography-mass spectrometry (GC-MS) for the monitoring of 21 phytoestrogens was developed and fully validated. Phytoestrogens were extracted by liquid-liquid extraction using methyl tert-butylether as the solvent. Food samples were enzymatically hydrolyzed with beta-glucuronidase/arylsulfatase. Before GC-MS, the analytes were derivatized to their trimethylsilyl derivatives by incubation with N,O-bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane for 30min at 80 degrees C. The calibration curves showed good linearity (r(2)>0.99) in the range of interest for each analyte. The limits of detection and quantification of the 21 phytoestrogens were in the ranges of 0.5-20 and 1-50ng/mL, respectively. The intra- and inter-day precisions were all below 15.7%. These multivariate procedures were efficient in determining the optimal conditions using peak heights as responses. English 대한화학회 GC-MS Analysis of Various Phytoestrogens in Health Functional Foods Article 10.1002/bkcs.11111 1 Bulletin of the Korean Chemical Society, v.38, no.4, pp.448 - 458 Bulletin of the Korean Chemical Society 38 4 448 458 N scie scopus kci ART002215488 000398836200007 2-s2.0-85016419641 Chemistry, Multidisciplinary Chemistry Article GAS CHROMATOGRAPHY/MASS SPECTROMETRY RANDOMIZED CONTROLLED-TRIAL TANDEM MASS-SPECTROMETRY LIQUID-CHROMATOGRAPHY HUMAN URINE POSTMENOPAUSAL WOMEN DIETARY-SUPPLEMENTS BISPHENOL-A DERIVATIZATION PROCEDURES ESTROGENIC ACTIVITY Phytoestrogen Health functional food Simultaneous Gas chromatography-mass spectrometry