Kim, Tae yeon Cho, Sukhyeong Woo, Han Min Lee, Sun-Mi Lee, Jinwon Um, Youngsoon Seo, Jin-Ho 2024-01-20T02:00:24Z 2024-01-20T02:00:24Z 2021-09-01 2017-04 0175-7598 https://pubs.kist.re.kr/handle/201004/122889 Conversion of crude glycerol derived from biodiesel processes to value-added chemicals has attracted much attention. Herein, Raoultella ornithinolytica B6 was investigated for the high production of 2,3-butanediol (2,3-BD) from glycerol without 1,3-propanediol (1,3-PD) formation, a by-product hindering 2,3-BD purification. By evaluating the effects of temperature, agitation speed, and pH control strategy, the fermentation conditions favoring 2,3-BD production were found to be 25 A degrees C, 400 rpm, and pH control with a lower limit of 5.5, respectively. Notably, significant pH fluctuations which positively affect 2,3-BD production were generated by simply controlling the lower pH limit at 5.5. In fed-batch fermentation under those conditions, R. ornithinolytica B6 produced 2,3-BD up to 79.25 g/L, and further enhancement of 2,3-BD production (89.45 g/L) was achieved by overexpressing homologous 2,3-BD synthesis genes (the budABC). When pretreated crude glycerol was used as a sole carbon source, R. ornithinolytica B6 overexpressing budABC produced 78.10 g/L of 2,3-BD with the yield of 0.42 g/g and the productivity of 0.62 g/L/h. The 2,3-BD titer, yield, and productivity values obtained in this study are the highest 2,3-BD production from glycerol among 1,3-PD synthesis-deficient 2,3-BD producers, demonstrating R. ornithinolytica B6 as a promising 2,3-BD producer from glycerol. English Springer Verlag High production of 2,3-butanediol from glycerol without 1,3-propanediol formation by Raoultella ornithinolytica B6 Article 10.1007/s00253-017-8094-y 1 Applied Microbiology and Biotechnology, v.101, no.7, pp.2821 - 2830 Applied Microbiology and Biotechnology 101 7 2821 2830 N scie scopus 000398705100014 2-s2.0-85009252478 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article KLEBSIELLA-OXYTOCA M1 CRUDE GLYCEROL FERMENTATION OPTIMIZATION CHEMICALS STRAIN 2,3-Butanediol Raoultella ornithinolytica Fed-batch fermentation budABC Crude glycerol