Bang, Kyeong-Mi Cho, Na Youn Kim, Won-Je Kim, Ae-Ryung Song, Hyun Kyu Kim, Eunice EunKyeong Kim, Nak-Kyoon 2024-01-20T02:00:44Z 2024-01-20T02:00:44Z 2021-09-01 2017-04 0253-2964 https://pubs.kist.re.kr/handle/201004/122909 Squamous cell carcinoma antigen recognized by T-cells 3 (SART3) is an essential recycling factor in premRNA splicing, which is required for association of U4/U6 small nuclear ribonucleoprotein (snRNP). SART3 contains two RNA recognition motifs (RRMs), and they are responsible for the tertiary interaction with U6 small nuclear RNA. Despite the importance of structural studies for understanding complicate U4/U6 snRNP recycling mechanism, only a few of them have been performed for SART3. Here, the structure of SART3 RRM2 was characterized by heteronuclear multi-dimensional nuclear magnetic resonance experiments. Nearly complete H-1, N-15, and C-13 chemical shifts of the backbone residues of RRM2 were assigned. In addition, the secondary structure of RRM2 were predicted by the chemical shift index and TALOS+ analyses, and the results showed that RRM2 forms a "beta(1)-alpha(1)-beta(2)-beta(3)-alpha(2)-beta(4)-beta(5)" structure, where beta(4) is not common in the canonical RRM domain structures. Our results will provide structural basis for investigation of SART3-mediated U4/U6 snRNP complex formation. English 대한화학회 Structural Characterization of RNA Recognition Motif-2 Domain of SART3 Article 10.1002/bkcs.11106 1 Bulletin of the Korean Chemical Society, v.38, no.4, pp.444 - 447 Bulletin of the Korean Chemical Society 38 4 444 447 N scie scopus kci ART002215487 000398836200006 2-s2.0-85013992044 Chemistry, Multidisciplinary Chemistry Article NMR CHEMICAL-SHIFTS U6 SNRNP PROTEIN SECONDARY STRUCTURE RECYCLING FACTOR FACTOR P110 PHOSPHORYLATION Squamous cell carcinoma antigen recognized by T-cells 3 Splicing RNA recognition motifs Nuclear magnetic resonance Structure