Jung, Kiwon An, Jun Min Eom, Dae-Woon Kang, Ki Sung Kim, Su-Nam 2024-01-20T02:00:46Z 2024-01-20T02:00:46Z 2021-09-01 2017-04 1226-8453 https://pubs.kist.re.kr/handle/201004/122911 Background: Fermented black ginseng (FBG) is processed ginseng by the repeated heat treatment and fermentation of raw ginseng. The protective effect and mechanism of FBG on cisplatin-induced nephrotoxicity was investigated to evaluate its therapeutic potential. Methods: The free radical scavenging activity of FBG was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH). In addition, the protective effect against cisplatin-induced renal damage was tested in rats. FBG was orally administered every day at a dose of 150 mg/kg body weight for 10 d, and a single dose of cisplatin was administered intraperitoneally (7.5 mg/kg body weight) with 0.9% saline on the 4th d. Results: The DPPH radical-scavenging activity of FBG (IC50 = 384 mu g/mL) was stronger than that of raw ginseng. The improved DPPH radical-scavenging activity was mediated by the generation phenolic compounds. The decreased cell viability by cisplatin was recovered significantly after treatment with FBG in a dose-dependent manner. Then, the protective effect of FBG on cisplatin-induced oxidative renal damage was investigated in rats. The decreased creatinine clearance levels, which are a reliable marker for renal dysfunction in cisplatin-treated rats, were reduced to the normal level after the administration of FBG. Moreover, FBG showed protective effects against cisplatin-induced oxidative renal damage in rats through the inhibition of NF-kappa B/p65, COX-2, and caspase-3 activation. Conclusion: These results collectively show that the therapeutic evidence for FBG ameliorates the nephrotoxicity via regulating oxidative stress, inflammation, and apoptosis. (C) 2017 The Korean Society of Ginseng, Published by Elsevier Korea LLC. English KOREAN SOC GINSENG DIABETIC RENAL DAMAGE OXIDATIVE STRESS PANAX-GINSENG RED GINSENG IN-VITRO ANTIOXIDANT APOPTOSIS INCREASE INFLAMMATION MEDICINE Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats Article 10.1016/j.jgr.2016.03.001 1 JOURNAL OF GINSENG RESEARCH, v.41, no.2, pp.188 - 194 JOURNAL OF GINSENG RESEARCH 41 2 188 194 scie scopus kci ART002214997 000400618000010 2-s2.0-85015541181 Plant Sciences Chemistry, Medicinal Integrative & Complementary Medicine Plant Sciences Pharmacology & Pharmacy Integrative & Complementary Medicine Article DIABETIC RENAL DAMAGE OXIDATIVE STRESS PANAX-GINSENG RED GINSENG IN-VITRO ANTIOXIDANT APOPTOSIS INCREASE INFLAMMATION MEDICINE cisplatin fermented black ginseng oxidative stress Panax ginseng renal damage